DNA replication: Flashcards
Every time a cell underogoes cell division what happens?
= All the dna is copied
= DNA replication
What happens in the first stage?
= enzyme DNA helicase attaches to the DNA molecule
= causes hydrogen bonds between the complementary base pairs to break
= causes 2 polynucleotide strands to seperate from eachother
What happens secondly?
= free nucleotides line up with their complementary bases on the DNa strands
= held into place by hydrogen bonds between the complementary bases
= not bonded by phosphodiester bonds
What are these free nucleotides called and what are they?
= activated nucleotides
= contains 3 phosphate heads
=
What happens thirdly?
= a 2nd ezyme attaches
= DNA polymerase
= moves down the molecule and catalyses the formation of a phosphodiester bond between the activated nucleotides
= this causes the activated nuclotides to loose their 2 other phosphate heads- as they leave it provides energy for the reaction
What are we now left with?
= two copies of double stranded dna molecule
= each contains one strand from the original DNA molecule+ brand new
= semi-conservative replication
What is a mutation?
= when an incorrect base is inserted into the growing polynucleotide strand causes the DNA sequence to change
= effects the organisms
= random and occur spontanously
=