ELIZA TESTS

Question 1

What are eliza tests based on?

Answer 1

= complementary and specific binding of an antigen to the antigen binding receptor of the antibodies
= ANTIGEN-ANTIBODY complex

= ability to produce monoclonal antibodies

Question 2

How does the eliza test work? (1)

Answer 2

= antigens are bounded to a test plate
= serum (blood, plasma) is added containing many antibodies
= wash off the excess antibodies

Question 3

How does the eliza test work? (2)

Answer 3

= second enzyme linked antibodies are added
= binds to the primary antibody
= rinsed to remove unbound antibodies

Question 4

How does the eliza test work? (3)

Answer 4

= substrate added
= causes colour change when substrate into product
= determines for positive and negative results