III - A. Virology | 2. Cultivation of viruses. Replication of viruses. The productive virus infection Flashcards
I. Replication of viruses
1A. What is the infective cycle of virus
I. Replication of viruses
1B. In the infective cycle, what happen in step 1: attachment?
attachment: interaction of virion with the host cell via receptor-ligand interactions
I. Replication of viruses
1C. In the infective cycle, what happen in step 2: penetration?
penetration: virus is taken up inside the cell (attachment initiates receptor-mediated endocytosis)
I. Replication of viruses
1D. In the infective cycle, what happen in step 3: uncoating?
uncoating: physical separation of the nucleic acid from the outer components of the virion
I. Replication of viruses
1E. In the infective cycle, what happen in step 3: „eclipse”?
„eclipse”: virion „disappeares”, time of viral genome replication and protein synthesis
- transcription of early genes, then translation into proteins
- replication of nucleic acid
- transcription of late genes, then translation into proteins
I. Replication of viruses
1F. In the infective cycle, what happen in step 5: release?
Release
* through lysis of host cell
* through budding
* througn exocytosis
I. Replication of viruses - Types of infection
2A. What are the 4 types of viral infection?
- productive infection
- abortive infection
- restrictive infection
- latent infection
I. Replication of viruses - Types of infection
2B. What is productive infection?
the virus replicates inside a susceptible cell (or permissive cell)
I. Replication of viruses - Types of infection
2C. What is abortive infection?
Abortive infection: cell were infected by the virus but can not produce any progeny virus as a result of the infection
I. Replication of viruses - Types of infection
2D. What is restrictive infection?
restrictive infection: the complete repertoire of virus genes necessary for virus replication is not transcribed and translated into functional products
=> the infection is referred to as restrictive
I. Replication of viruses - Types of infection
2F. What is latent infection?
latent infection: the residence in the body of a specific infectious agent without any manifest symptoms
I. Replication of viruses
3A. What are the infection types based on clinical symptoms?
- acute
- chronic (or persistent)
- latent-reactivating
- slow virus infection
I. Replication of viruses
3B. Give examples of viruses causing acute infection
- Rhinovirus
- Rotavirus
- Influenza virus
I. Replication of viruses
3C. Give examples of viruses causing persistent infection
Lymphocytic choriomeningitis virus
I. Replication of viruses
3D. Give examples of virus causing latent infection
Herpes simplex virus