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AA Microbio Semifinal > IIA. Systemic Bacteriology | 7. Escherichia coli. > Flashcards

IIA. Systemic Bacteriology | 7. Escherichia coli. Flashcards

1
Q

I. Basics - The microflora of the digestive system
2. Describe microflora of the digestive system in the UPPER PART

A
  • poor in bacteria (101-102/g in stomach)
  • more than 104 CFU/g if the stomach pH is higher than 2
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1
Q

I. Basics - The microflora of the digestive system
1. What is The microflora of the digestive system influenced?

A
  • throat flora
  • the flora of the saliva
  • food
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2
Q

I. Basics - The microflora of the digestive system
3. Describe microflora of the digestive system in the Duodenum

A
  • 103-104 CFU/g
  • Streptococcus sp. and Lactobacillus sp.
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3
Q

I. Basics - The microflora of the digestive system
1. Describe microflora of the digestive system in the UPPER PART

A
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4
Q

I. Basics - The microflora of the digestive system
4. Describe microflora of the digestive system in the Ileum

A
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5
Q

I. Basics - The microflora of the digestive system
5A. Describe microflora of the digestive system in the Large intestine

A
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6
Q

I. Basics - The microflora of the digestive system
5B. What are obligate anaerobes in the Large intestine

A
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7
Q

I. Basics - The microflora of the digestive system
5C. What are Facultative anaerobes in the Large intestine

A
  • Enterococcus
  • Enterobacteriaceae
  • E. coli: only ~ 1% !
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8
Q

I. Basics
6. What is the role of normal GI flora?

A
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9
Q

I. Basics
7A. Explain Colonisation of the infants

A
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10
Q

I. Basics
7B. What are the features of Caesarean section?

A
  • Delayed colonisation
  • Might be gastrointestinal and immunological problems in the future!
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11
Q

I. Basics
7C. What are the Influencing factors in Colonisation of the infants?

A
  • Mother’s dietary intake
  • Breast feeding – Bifidobacterium versus bottle feeding – adult flora
  • Premature infants, etc.
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12
Q

I. Basics
8. What are the reasons for Imbalance of the GI normal flora?

A
  • dietary changes
  • wide spectrum antibiotic
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13
Q

I. Basics
9. What is the result of Imbalance of the GI normal flora?

A
  • absorption problems
  • hypovitaminosis
  • peristaltic problems
  • increased gas production
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14
Q

I. Basics
10. What is the Antibiotic effect

A
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15
Q

II. Enterobacteriaceae family
1. What are the features of Enterobacteriaceae family?

A
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16
Q

II. Enterobacteriaceae family
2. What is the morphology of Enterobacteriaceae family?

A

2-4 µm rod

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17
Q

II. Enterobacteriaceae family
3. What do we cultivate Enterobacteriaceae family on?

A

Cultivation on selective culture medium

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18
Q

II. Enterobacteriaceae family
4. What are Biochemical tests used for identification?

A
19
Q

II. Enterobacteriaceae family
5. Tick this table

A
20
Q

II. Enterobacteriaceae family
7. Make a schematic diagram of gram-negative rods facultative anaerobic attack sugars by fermentation oxidase-negative

A
21
Q

II. Enterobacteriaceae family
8. Make a schematic diagram of Enterobacteriaceae

A
22
Q

II. Enterobacteriaceae family
9. What are the Two main types of infectious disease associated with Enterobacteriaceae?

A
  • intestinal diseases
  • extraintestinal diseases
23
Q

II. Enterobacteriaceae family
10. What are some human infections caused by Enterobacteriaceae family?

A
24
Q

II. Enterobacteriaceae family
11A. What are features of Extra intestinal infections?

A
25
Q

II. Enterobacteriaceae family
12. What are features of Urinary tract infections (UTI)?

A
26
Q

II. Enterobacteriaceae family
13. What are the features of nosocomial infections?

A
27
Q

II. Enterobacteriaceae family
14. What are the features of ESBL?

A
28
Q

II. Enterobacteriaceae family
15. What are the features of MBLs?

A
  • Metallo-beta lactamases
  • Resistance to all β-lactams, including carbapenems !
  • Klebsiella, Pseudomonas, Acinetobacter
29
Q

II. Enterobacteriaceae family
16. What is the Treatment of Enterobacteriaceae infections?

A
30
Q

III. Escherichia coli
1. What are the features of Escherichia coli?

A
31
Q

III. Escherichia coli
2. What is the morphology of E.coli?

A

Morphology: 2-4 µm, flagellated rod

32
Q

III. Escherichia coli
3. What is the staining of E.coli?

A

Gram-negative

33
Q

III. Escherichia coli
4. How should we cultivate Escherichia coli?

A

normal agar, EMB agar

34
Q

III. Escherichia coli
5. What are the biochemical properties of Escherichia coli?

A

1/ glucose +
2/ lactose +
3/ indol +
4/ urease –
5/ H2S –

35
Q

III. Escherichia coli
5. Describe the antigenic structure?

A

„O” >180
„H” 55
„K” 100

36
Q

III. Escherichia coli
6. What are the intestinal infections caused by E.coli?

A
  • EPEC enteropathogenic E. coli dyspepsia coli – colitis of the newborns
  • ETEC enterotoxic E.coli – cholera like diarrhea, „traveler’s disease”
  • EIEC enteroinvasive E. coli - dysentery
  • EHEC enterohaemorrhagic E.coli, dysenteriform diarrhea
  • Enteroaggregative E. coli - EAEC
37
Q

III. Escherichia coli
7. What are the features of Enteropathogenic E. coli - EPEC?

A
  • Attachment to the epithel cells of the small intestine
  • Children < 1 year
  • „dyspepsiacoli”
  • Outbreaksinnursing homes
38
Q

III. Escherichia coli
8A. What are the features of Enterotoxin producing E. coli - ETEC?

A
39
Q

III. Escherichia coli
8B. What is the therapy for Enterotoxin producing E. coli - ETEC?

A

antibiotics, fluid replacement

40
Q

III. Escherichia coli
9A. What are the features of Enterohaemorrhagic E. coli - EHEC?

A
  1. Shiga-like toxin or Verotoxin from Shigella on plasmid
  2. Severe, bloody enteritis, without fever
  3. Maybe HUS
  4. Contaminated beef + milk
41
Q

III. Escherichia coli
9A. What is the therapy of Enterohaemorrhagic E. coli - EHEC?

A

antibiotics, fluid replacement

42
Q

III. Escherichia coli
10A. What are the features of Enteroinvasive E. coli - EIEC?

A
43
Q

III. Escherichia coli
10B. What is the transmission of Enteroinvasive E. coli - EIEC?

A

– contact spread
– less easy than Shigella

44
Q

III. Escherichia coli
11A. What are the features of Enteroaggregative E. coli - EAEC?

A
45
Q

III. Escherichia coli
12. What are the features of Neonatal menigitis caused by Escherichia coli?

A
46
Q

III. Escherichia coli
13. Look at this picture and explain E.coli

A

AA Microbio Semifinal (99 decks)

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