Bacterial genetics 1/12/17 Mulvey TEST #1

Question 1

What is a gene referred to as in bacteria?

Answer 1

-Cistron

Question 2

T/F

Bacteria can be polycistronic

Answer 2

True

Question 3

In a polycistronic gene how many mRNAs are made?

Answer 3

-One with multiple genes

Question 4

If you have a single transcription factor that can effect multiple genes/cistrons what is that called ?

Answer 4

-Regulon

Question 5

When what is bound in bacteria genes can no longer be made?

Answer 5

-DtxR

Question 6

T/F

Transcription and Translation are never coupled in bacteria.

Answer 6

False

They are coupled

Question 7

What do sigma factors do?

Answer 7

-Recognize specific promoter sequences and direct the RNA polymerase complex where to bind within DNA

Question 8

What do bacteria contain that mediate respones to diverse stresses?

Answer 8

-Sigma factors

Question 9

What are two ways the bacteria obtain diversity?

Answer 9

• Mutation

- Horizontal Gene Exchange Mechanisms

Question 10

What can point mutation impact?

Answer 10

-Expression of virulence factors and the sensitivity of antibiotic targets

Question 11

What are there types of Horizontal gene transfer?

Answer 11

• Bacterial transformation (cell dies and releases DNA)
• Bacterial transduction (Phage picks up DNA from another bacteria)
• Bacterial conjugation (Plasmid transfer between bacteria)