BMS02-1009 Enzymes & Coenzymes

Question 1

What is an enzyme?

Answer 1

Biological catalyst speeding up ROR without altering eqb

Question 2

Will an enzyme act on both optical isomers?

Answer 2

Not normally

Question 3

The 6 classes of enzymes with examples

Answer 3

Oxidoreductases e.g. lactate dehydrogenase
Ligases e.g. DNA ligase
Isomerases e.g. phosphoglucose isomerase
Lyases e.g. ATP citrate lyase
Hydrolases e.g. trypsin
Transferases e.g. alanine aminotransferase

Question 4

What do oxidoreductases do?

Answer 4

Transfer protons and electrons

Question 5

What do transferases do?

Answer 5

Transfer functional groups

Question 6

What do lyases do?

Answer 6

Adding groups to double bonds or making double bonds by removing groups

Question 7

What do hydrolases do?

Answer 7

Adding water

Question 8

What do isomerases do?

Answer 8

Trasfers functional groups in the same molecule

Question 9

What do ligases do?

Answer 9

Use ATP to form new covalent bonds

Question 10

List some inorganic cofactors

Answer 10

Iron in catalase

Potassium in pyruvate kinase

Question 11

List some organic cofactors

Answer 11

NAD
FAD
ATP

Question 12

What is an isoenzyme and give an example of one

Answer 12

Difference enzymes that do the same reaction normally found in different compartments of the cell
lactate dehydrogenase in muscle and heart