Lab practical 2 short answers

Question 1

The fire extinguisher is out the side door and past the what? (two words)

Answer 1

beverage cart

Question 2

The fire extinguisher is next to the what?

Answer 2

stairwell

Question 3

How do you spell the type of arrangement that is long and has spirals?

Answer 3

spirochetes

Question 4

How do you spell the type of arrangement that is short and stocky and spirals?

Answer 4

spirilum

Question 5

What is the cell shape of yeasts?

Answer 5

Oval

Question 6

Yeasts either have “no arrangement” OR they have what type of arrangement?

Answer 6

pseudohyphae

Question 7

Are yeasts much bigger than bacteria?

Answer 7

Yes

Question 8

What do yeasts have in their cell walls?

Answer 8

Chitan

Question 9

What will not work because of the chitan in the cell wall of yeast? (two words)

Answer 9

gram stain

Question 10

What is an example of a gram positive cell envelope?

Answer 10

Staphylococcus aureus

Question 11

What is an example of a gram negative cell envelope?

Answer 11

Escherichia coli

Question 12

What is an example of an acid fast cell envelope?

Answer 12

Mycobacterium tuberculosis

Question 13

What is an example of an Endospore former and capsule former?

Answer 13

Bacillus anthracis

Question 14

We use acid fast on what type of bacteria?

Answer 14

mycobacterium

Question 15

What is used for the primary stain when performing acid fast stains?

Answer 15

methylene blue

Question 16

What do we use in acid fast stains instead of regular alcohol?

Answer 16

acid alcohol

Question 17

Is heat involved with acid fast staining?

Answer 17

Yes

Question 18

What do we use after the acid alcohol and before the counterstain?

Answer 18

Heat

Question 19

What is used as the counterstain in acid staining?

Answer 19

carbofusion

Question 20

What material to we use in acid fast staining that makes us call it “acid fast”?

Answer 20

acid alcohol

Question 21

Why do we use alcohol in gram stain?

Answer 21

to break down the cell wall to drive the dye in

Question 22

What happens if we use acid alcohol on a gram stain instead of the normal decolorizer of ethyl alchol? (two words)

Answer 22

completely decolorize

Question 23

What is the fundamental difference of the material contained in acid fast cell envelopes?

Answer 23

mycolic acid

Question 24

In one word, how would you describe a cell wall containing mycolic acid?

Answer 24

waxy

Question 25

What is a medically significant acid fast cell?

Answer 25

Mycobacterium tuberculosis

Question 26

What is the primary stain for spore staining?

Answer 26

malachite green

Question 27

What is the counter stain for spore staining?

Answer 27

safranin

Question 28

Is it easy for the spores to survive in infection?

Answer 28

Yes

Question 29

What is needed for the body to kill spores?

Answer 29

Heat the body so high

Question 30

What would also be killed if we heat the body high enough to kill the spores? (one word)

Answer 30

body

Question 31

What is the relevance of spores in microbiology laboratory sterility? (three words)

Answer 31

Hard to kill

Question 32

Where do we use the spores in microbiology laboratory sterility?

Answer 32

autoclave

Question 33

What are the components of capsule stain?

Answer 33

India ink

Question 34

What is the function of india ink in the process of capsule stain?

Answer 34

simple

Question 35

Is India ink in a capsule stain a positive stain or negative stain?

Answer 35

negative

Question 36

What is rendered ineffective by capsules in infection (not environment)?

Answer 36

phagocytosis

Question 37

What happened if the temperature goes up on cell function?

Answer 37

enzymes denature

Question 38

What cannot be reached if the temperature goes down on cell function?

Answer 38

activation energy

Question 39

What type of temperature do Thermophiles like?

Answer 39

heat

Question 40

What type of temperature do Pschryophiles like?

Answer 40

cold

Question 41

What type of temperature do Mesophiles like? (one word)

Answer 41

body

Question 42

What are the range of degrees in celsius that thermophiles survive in? (x to x)

Answer 42

20 to 70

Question 43

What is the ideal number of degrees in celsius that thermophiles thrive?

Answer 43

50

Question 44

What are the range of degrees in celsius that psychrophiles survive in?(x to x)

Answer 44

0 to 20

Question 45

What is the ideal number of degrees in celsius that psychrophiles thrive in?

Answer 45

15

Question 46

What is the range of degrees in celsius that mesophiles survive in? (x to x)

Answer 46

20 to 40

Question 47

What is the ideal number of degrees in celsius that mesophiles thrive in?

Answer 47

37

Question 48

There are three ways spores are relevant in bacterial survival in the environment. In the first way, the spores PROTECT AGAINST what? (two words)

Answer 48

drying out

Question 49

There are three ways spores are relevant in bacterial survival in the environment. In the second way, the spores ACT AS A what? (two words)

Answer 49

food reserve

Question 50

The reasons the spores can act as a food reserve is because they have something that can be eaten - what is it that can be eaten? (three words)

Answer 50

long chain sugars

Question 51

There are three ways spores are relevant in bacterial survival in the environment. In the third way, the spores PROTECT AGAINST what?

Answer 51

radiation