3.3.7 Electrolytes (Phosphate) Flashcards
Phosphate is a major Intracellular ________
anion
Phosphate is the component of several essential biomolecules such as
- ATP
- ADP
- 2,3 DPG
- DNA
- RNA
- Phospholipids
T or F
T
Phosphate is usually measured as organic phosphorus
T or F
F
Inorganic
Ref values of Phosphate:
Normal:
a. 2.4–4.4 mg/dL
b. 1.4–4.4 mg/dL
c. 3.4–4.4 mg/dL
a
Panic value for Phosphorus?
hypophosphatemia: ___
hyperphosphatemia: ___
a. ≤ 1 ; ≥ 8.9 mg/dL
b. ≤ 2 ; ≥ 5.9 mg/dL
c. ≤ 9 ; ≥ 1.9 mg/dL
a
Also known as ammonium molybdate method which phosphate is made to react to it
a. Fiske-Subbarow
b. Ammonium phosphomolyndate
a
Final product for Fiske-Subbarow?
a. Ammonium molybdate
b. Ammonium phosphomolybdate
a
Detection techniques for Phosphate:
Stop at Ammonium phosphomolybdate then measure the absorbance of phosphomolybdate at 340 nm (INCREASED absorbance; UV measurement)
T or F
T
Detection techniques for Phosphate:
Convert it to a colorimetric method – from UV, convert it by using a reducing agent Such as?
a. Ascorbic acid
b. H2O2
a
Detection techniques for Phosphate:
The reducing agent will reduce Ammonium phosphomolybdate into phosphomolybdenum ______ (colorimetric)
a. Blue
b. Red
a
Detection techniques for Phosphate:
Determine the increase in absorbance at
a. 600-700 nm
b. 450-600 nm
c. 600-750 nm
d. 500-700 nm
a
Diabetic Ketoacidosis
a. Hypophosphatemia
b. Hyperphosphatemia
a (Increase EXCRETION)
Long term TPN, IBD, alcoholism
a. Hypophosphatemia
b. Hyperphosphatemia
a (Decreased Intake)
Renal Failure
a. Hypophosphatemia
b. Hyperphosphatemia
b (Decrease Excretion)
Increased intake of Milk or Laxatives
a. Hypophosphatemia
b. Hyperphosphatemia
b
