3.2.1.2 Clinical Enzymology (Creatine Kinase, CK determination) Flashcards
CK Specimen consideration includes:
- Avoid prolonged storage
- Avoid Hemolysis
- Avoid using chelating agents
Which of the following does not belong?
Avoid hemolysis
Which among the 3 types of CK is the most labile?
CK1
Reverse reaction
a. Tanzer-Gilvard
b. Oliver-Rosalki
b
Forward reaction
a. Tanzer-Gilvard
b. Oliver-Rosalki
a
Creatinine + ATP – (CK)–> Creatinine PO4 + ADP
a. Tanzer-Gilvard
b. Oliver-Rosalki
a
Creatinine PO4 + ADP– (CK)–> Creatinine + ATP
a. Tanzer-Gilvard
b. Oliver-Rosalki
b
Pyruvate + NADH <-(LDH)-> lactate + (NAD)
a. Tanzer-Gilvard
b. Oliver-Rosalki
a
G6P + NADP <-(G6PD)-> 6PG+ NADPH
a. Tanzer-Gilvard
b. Oliver-Rosalki
b
How is NAD being measured?
a. Decrease absorbance at 340nm
b. Increase absorbance at 340nm
a
How is NADPH being measured?
a. Decrease absorbance at 340nm
b. Increase absorbance at 340nm
b
Reference value:
15 - 160 U/L
a. Male
b. Female
a
Reference value:
15 - 130 U/L
a. Male
b. Female
b