2.2.6 Plasma Lipid Analysis (Analytical Method, Cholesterol) Flashcards
The following are chemical methods for cholesterol except?
a. Libermann-Buchard
b. Salkowski
c. Modified Abell-Kendall
d. All of the above
e. None of the above
e
Cholesterol ester + H2O –(Alcoholic KOH)—> cholesterol + fatty acid
what is the process?
a. Saponification
b. Extraction
c. Purification
d. Colorimetry
a
Uses ferric chloride or ions as reagent
a. Liebermann-Buchardy
b. Salkowski
c. Modified Abell-Kendall
b
Uses Acetic anhydride as reagent
a. Liebermann-Buchardy
b. Salkowski
c. Modified Abell-Kendall
a
N-hexane or petroleum ether is the reagent used instead of Bloor’s reagent
3 step method, Reference method of CDC
a. Liebermann-Buchardy
b. Salkowski
c. Modified Abell-Kendall
c
Colorimetry
a. Liebermann-Buchardy
b. Salkowski
Both
Color of Cholestadienly MSA?
Green
Color of Cholestadienyl DSA?
Red
Chlestadienyl MSA
a. Liebermann-Buchardy
b. Salkowski
c. Modified Abell-Kendall
a
Cholestadienly DSA
a. Liebermann-Buchardy
b. Salkowski
c. Modified Abell-Kendall
b
Alcoholic potassium hydroxide
a. Saponification
b. Extraction
c. Purification
d. Colorimetry
a
Digitonin
a. Saponification
b. Extraction
c. Purification
d. Colorimetry
c
Bloor’s reagent (Ethanol-ether)
a. Saponification
b. Extraction
c. Purification
d. Colorimetry
b
H2SO4, acetic anhydride or ferric ions
a. Saponification
b. Extraction
c. Purification
d. Colorimetry
d
Removal of protein interference
a. Saponification
b. Extraction
c. Purification
d. Colorimetry
b
Hydrolysis of cholesterol esters
a. Saponification
b. Extraction
c. Purification
d. Colorimetry
a
Precipitation of free cholesterol
a. Saponification
b. Extraction
c. Purification
d. Colorimetry
c
Formation of colored compound
a. Saponification
b. Extraction
c. Purification
d. Colorimetry
d
The enzyme that is used in the Enzymatic method for Cholesterol (Final product is Cholestenone + H2O2)
a. Cholesterol esterase
b. Cholesterol oxidase
c. Cholesterol lipase
d. Cholesterol hydrolase
b
The enzyme that is used in the Enzymatic method for Cholesterol (Final product is Cholesterol + Fatty acid)
a. Cholesterol esterase
b. Cholesterol oxidase
c. Cholesterol lipase
d. Cholesterol hydrolase
a
Reference value for Cholesterol:
Desirable: <200 mg/dl
Borderline high: 200 - 239 mg/dl
High: More than or equal to 240 mg/dl
T or F
T
Cholesterol cutoff points according to age:
Moderate risk: > or equal to 220 mg/dl
High Risk: > or equal to 240 mg/dl
a. 2-19
b. 20-29
c. 30-39
d. 40+
c
Cholesterol cutoff points according to age:
Moderate risk: > or equal to 200 mg/dl
High Risk: > or equal to 220 mg/dl
a. 2-19
b. 20-29
c. 30-39
d. 40+
b
Cholesterol cutoff points according to age:
Moderate risk: > or equal to 170 mg/dl
High Risk: > or equal to 185 mg/dl
a. 2-19
b. 20-29
c. 30-39
d. 40+
a
Cholesterol cutoff points according to age:
Moderate risk: > or equal to 240 mg/dl
High Risk: > or equal to 260 mg/dl
a. 2-19
b. 20-29
c. 30-39
d. 40+
d
Risk of high cholesterol is CHD
T or F
T
Clinical significance of abnormal results for cholesterol:
- Fredrickson: 2a, 2b, 3
- Nephrotic syndrome
- Poorly controlled diabetes mellitus
- Hypothyroidism
a. Hypercholesterolemia
b. Hypocholesterolemia
a
Clinical significance of abnormal results for cholesterol:
- Severe liver disease
- Malnutrition and malabsorption
- Hyperthyroidsm
a. Hypercholesterolemia
b. Hypocholesterolemia
b
