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MTAP 1 Clinical Chemistry > 2.2.6 Plasma Lipid Analysis (Analytical Method, Cholesterol) > Flashcards

2.2.6 Plasma Lipid Analysis (Analytical Method, Cholesterol) Flashcards

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1
Q

The following are chemical methods for cholesterol except?

a. Libermann-Buchard
b. Salkowski
c. Modified Abell-Kendall
d. All of the above
e. None of the above

A

e

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2
Q

Cholesterol ester + H2O –(Alcoholic KOH)—> cholesterol + fatty acid

what is the process?

a. Saponification
b. Extraction
c. Purification
d. Colorimetry

A

a

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3
Q

Uses ferric chloride or ions as reagent

a. Liebermann-Buchardy
b. Salkowski
c. Modified Abell-Kendall

A

b

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4
Q

Uses Acetic anhydride as reagent

a. Liebermann-Buchardy
b. Salkowski
c. Modified Abell-Kendall

A

a

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5
Q

N-hexane or petroleum ether is the reagent used instead of Bloor’s reagent

3 step method, Reference method of CDC

a. Liebermann-Buchardy
b. Salkowski
c. Modified Abell-Kendall

A

c

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6
Q

Colorimetry

a. Liebermann-Buchardy
b. Salkowski

A

Both

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7
Q

Color of Cholestadienly MSA?

A

Green

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8
Q

Color of Cholestadienyl DSA?

A

Red

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9
Q

Chlestadienyl MSA

a. Liebermann-Buchardy
b. Salkowski
c. Modified Abell-Kendall

A

a

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10
Q

Cholestadienly DSA

a. Liebermann-Buchardy
b. Salkowski
c. Modified Abell-Kendall

A

b

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11
Q

Alcoholic potassium hydroxide

a. Saponification
b. Extraction
c. Purification
d. Colorimetry

A

a

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12
Q

Digitonin

a. Saponification
b. Extraction
c. Purification
d. Colorimetry

A

c

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13
Q

Bloor’s reagent (Ethanol-ether)

a. Saponification
b. Extraction
c. Purification
d. Colorimetry

A

b

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14
Q

H2SO4, acetic anhydride or ferric ions

a. Saponification
b. Extraction
c. Purification
d. Colorimetry

A

d

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15
Q

Removal of protein interference

a. Saponification
b. Extraction
c. Purification
d. Colorimetry

A

b

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16
Q

Hydrolysis of cholesterol esters

a. Saponification
b. Extraction
c. Purification
d. Colorimetry

A

a

17
Q

Precipitation of free cholesterol

a. Saponification
b. Extraction
c. Purification
d. Colorimetry

A

c

18
Q

Formation of colored compound

a. Saponification
b. Extraction
c. Purification
d. Colorimetry

A

d

19
Q

The enzyme that is used in the Enzymatic method for Cholesterol (Final product is Cholestenone + H2O2)

a. Cholesterol esterase
b. Cholesterol oxidase
c. Cholesterol lipase
d. Cholesterol hydrolase

A

b

20
Q

The enzyme that is used in the Enzymatic method for Cholesterol (Final product is Cholesterol + Fatty acid)

a. Cholesterol esterase
b. Cholesterol oxidase
c. Cholesterol lipase
d. Cholesterol hydrolase

A

a

21
Q

Reference value for Cholesterol:

Desirable: <200 mg/dl
Borderline high: 200 - 239 mg/dl
High: More than or equal to 240 mg/dl

T or F

A

T

22
Q

Cholesterol cutoff points according to age:

Moderate risk: > or equal to 220 mg/dl
High Risk: > or equal to 240 mg/dl

a. 2-19
b. 20-29
c. 30-39
d. 40+

A

c

23
Q

Cholesterol cutoff points according to age:

Moderate risk: > or equal to 200 mg/dl
High Risk: > or equal to 220 mg/dl

a. 2-19
b. 20-29
c. 30-39
d. 40+

A

b

24
Q

Cholesterol cutoff points according to age:

Moderate risk: > or equal to 170 mg/dl
High Risk: > or equal to 185 mg/dl

a. 2-19
b. 20-29
c. 30-39
d. 40+

A

a

25
Q

Cholesterol cutoff points according to age:

Moderate risk: > or equal to 240 mg/dl
High Risk: > or equal to 260 mg/dl

a. 2-19
b. 20-29
c. 30-39
d. 40+

A

d

26
Q

Risk of high cholesterol is CHD

T or F

A

T

27
Q

Clinical significance of abnormal results for cholesterol:

  • Fredrickson: 2a, 2b, 3
  • Nephrotic syndrome
  • Poorly controlled diabetes mellitus
  • Hypothyroidism

a. Hypercholesterolemia
b. Hypocholesterolemia

A

a

28
Q

Clinical significance of abnormal results for cholesterol:

  • Severe liver disease
  • Malnutrition and malabsorption
  • Hyperthyroidsm

a. Hypercholesterolemia
b. Hypocholesterolemia

A

b

MTAP 1 Clinical Chemistry (97 decks)

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