3.2.5.2 Clinical Enzymology (Pancreatic enzymes, Amylase determination)

Question 1

Specimen consideration for Amylase includes:

• Avoid intake of pain relievers
• Avoid use of lipemic serum
• Avoid hemolysis

Which of the following does not belong?

Answer 1

Avoid hemolysis

Question 2

Pain relievers induce microsomal release of salivary amylase

T or F

Answer 2

T

Question 3

Decrease in color of starch-iodine complex

a. Amyloclastic method
b. Saccharogenic method
c. Chromogenic method
d. Coupled enzymatic method

Answer 3

a

Question 4

Increase color intensity of soluble dye-substrate solution

a. Amyloclastic method
b. Saccharogenic method
c. Chromogenic method
d. Coupled enzymatic method

Answer 4

c

Question 5

Increase reducing sugars as starch is hydrolyzed by AMS (Classic reference method)

a. Amyloclastic method
b. Saccharogenic method
c. Chromogenic method
d. Coupled enzymatic method

Answer 5

b

Question 6

Increase absorbance due to NADH

a. Amyloclastic method
b. Saccharogenic method
c. Chromogenic method
d. Coupled enzymatic method

Answer 6

d

Question 7

Reference method for amylase

25-130 U/L

a. serum
b. urine

Answer 7

a

Question 8

Reference method for amylase

1-15 U/L

a. serum
b. urine

Answer 8

b