2.4.2 Proteins (Protein electrophoresis) Flashcards
Sample used in protein electrophoresis?
Serum
Serum samples are applied close to the ________ end of a support medium that is saturated with an alkaline buffer (pH ____) at which pH proteins carry a ________ charge and migrate toward the _______ (+ electrode)
Cathode
8.6
negative
Anode
All of this are supporting media for protein electrophoresis except:
a. Agarose
b. Cellulose acetate
c. Polyacrylamide gel
d. Acrylic gel
d
Stains used in Protein Electrophoresis include:
- Coomassie Brilliant Blue
- Poncean S
- Amido Black
- Lissamine Green
- Creva’s orange
Which of the following does not belong?
Creva’s orange
Specialized colorimeter designed to scan and quantitate electrophoretic pattern
a. Densitometer
b. Turbidimetry
c. Nephelometry
d. Spectrophotometry
a
Uses a higher voltage coupled with a cooling system and a more concentrated buffer; separates proteins into 12 or more bands; detects unusual monoclonal bands
a. Low-resolution protein electrophoresis
b. High-resolution protein electrophoresis
c. Beta electrophoresis
d. Monoclonal electrophoresis
b
Proteins are amphoteric (can react both base and acid)
T or F
T
Protein electrophoresis Direction:
Cathode to anode (+)
T or F
T
Anode is applied near the Origin
T or F
F
Cathode is applied near the origin
What is the purpose of stain in Protein electrophoresis?
a. Distinguish cell
b. Inhibits protein
c. Make bands visible
d. Make electrode visible
c
Staining the bands provides a Quantitative result
T or F
F
Qualitative since stain can only give the intensity and visibility of this bands
The application of detection technique that must be used which make it quantitative is called?
a. Read out device
b. Densitometry
c. Turbidimetry
d. Electrophoresis
b
Densitometer can detect ___ major bands
a. 1
b. 3
c. 5
d. 7
c
The 5 major bands are?
Albumin
alpha 1 and 2
Beta
Gamma
The most darkly stained and most anodal Fraction is _______
Albumin
