2.4.2 Proteins (Protein electrophoresis)

Question 1

Sample used in protein electrophoresis?

Answer 1

Serum

Question 2

Serum samples are applied close to the ________ end of a support medium that is saturated with an alkaline buffer (pH ____) at which pH proteins carry a ________ charge and migrate toward the _______ (+ electrode)

Answer 2

Cathode

8.6

negative

Anode

Question 3

All of this are supporting media for protein electrophoresis except:

a. Agarose
b. Cellulose acetate
c. Polyacrylamide gel
d. Acrylic gel

Answer 3

d

Question 4

Stains used in Protein Electrophoresis include:

• Coomassie Brilliant Blue
• Poncean S
• Amido Black
• Lissamine Green
• Creva’s orange

Which of the following does not belong?

Answer 4

Creva’s orange

Question 5

Specialized colorimeter designed to scan and quantitate electrophoretic pattern

a. Densitometer
b. Turbidimetry
c. Nephelometry
d. Spectrophotometry

Answer 5

a

Question 6

Uses a higher voltage coupled with a cooling system and a more concentrated buffer; separates proteins into 12 or more bands; detects unusual monoclonal bands

a. Low-resolution protein electrophoresis
b. High-resolution protein electrophoresis
c. Beta electrophoresis
d. Monoclonal electrophoresis

Answer 6

b

Question 7

Proteins are amphoteric (can react both base and acid)

T or F

Answer 7

T

Question 8

Protein electrophoresis Direction:

Cathode to anode (+)

T or F

Answer 8

T

Question 9

Anode is applied near the Origin

T or F

Answer 9

F

Cathode is applied near the origin

Question 10

What is the purpose of stain in Protein electrophoresis?

a. Distinguish cell
b. Inhibits protein
c. Make bands visible
d. Make electrode visible

Answer 10

c

Question 11

Staining the bands provides a Quantitative result

T or F

Answer 11

F

Qualitative since stain can only give the intensity and visibility of this bands

Question 12

The application of detection technique that must be used which make it quantitative is called?

a. Read out device
b. Densitometry
c. Turbidimetry
d. Electrophoresis

Answer 12

b

Question 13

Densitometer can detect ___ major bands

a. 1
b. 3
c. 5
d. 7

Answer 13

c

Question 14

The 5 major bands are?

Answer 14

Albumin

alpha 1 and 2

Beta

Gamma

Question 15

The most darkly stained and most anodal Fraction is _______

Answer 15

Albumin

Question 16

The greater the intensity of staining, the taller the peak in densitometer

T or F

Answer 16

T

Question 17

High resolution electriophoresis can detect more than _________ bands

a. 5
b. 5-11
c. 5-12
d. 5-13

Answer 17

c

Question 18

high resolution electrophoresis uses ________, and a cooling system which can detect up to 5-12 bands

a. High voltage
b. Concentrated buffer
c. Both

Answer 18

c