2.4.6 Proteins (Lab determination of Proteins) Flashcards
Measures Refractive index due to proteins in solution
a. Kjeldahl Method
b. Refractometry Method
c. Turbidimetry
d. UV absorption
b
Ultimate reference method based on measuring nitrogen content
a. Kjeldahl Method
b. Refractometry Method
c. Turbidimetry
d. UV absorption
a
Change in optical density followin precipitation with TRICHLOROACETIC ACID (TCA, Sulfosalicylic acid (SSA), Benzalkonium chlorinde
a. Kjeldahl Method
b. Refractometry Method
c. Turbidimetry
d. UV absorption
c
UV absorption:
Measurement of absorbance at _____ owing mostly to Tryptophan, Tyrosine, and Phenylalanine
280 nm
Biuret method:
Detection of peptide bonds, forming a _______ which is measured at _____ nm
a. Violet, 540nm
b. Blue, 500nm
c. Green, 400nm
d. Red, 700nm
a
Biuret method which is a colorimetric method has a reagent that comprises of:
- Copper sulfate
- Rochelle salt
- NaOH
- Potassium iodide
Which of the following does not belong?
None
Oxidation of phenolic groups
a. Folin-Ciocalteau (Lowry)
b. Ninhydrin Method (Colorimetric)
c. Dye-binding technique
a
Lowry method oxidized phenolic groups producing what color?
a. deep Violet
b. deep Blue
c. deep Red
d. deep Green
b
Lowry is more sensitive than biuret
T or F
T
Formation of VIOLET color in the presence of AMINO acid
a. Folin-Ciocalteau (Lowry)
b. Ninhydrin Method (Colorimetric)
c. Dye-binding technique
b
Dye-binding technique
What is the dye used for total protein?
a. Coomassie Brilliant blue
b. Ponceus S
c. Amido black
d. Methyl Orange
a
Dye used for Albumin
a. Coomassie Brilliant blue
b. Ponceus S
c. Amido black
d. Methyl Orange
d
(Nonspecific)
The following other dyes is used in the detection of albumin:
- 2,4 HABA
- BCG
- BCP
Which among the following is most common
BCG
The following other dyes is used in the detection of albumin:
- 2,4 HABA
- BCG
- BCP
Which among the following is specific, sensitive and precise?
BCP
The following other dyes is used in the detection of albumin:
- 2,4 HABA
- BCG
- BCP
Which among the following is subject to interference
2,4 HABA
Reference Values:
6.5 - 8.3 g/dL
a. TP, Ambulatory
b. Albumin
a
Reference Values:
3.5 - 5.5 g/dL
a. TP, Ambulatory
b. Albumin
b
A/G Ratio = A/(TP-A)
What is the normal value?
a. 1.1 - 2.0
b. 1.0 - 2.1
c. 1.1 - 2.1
a
If low/inverted ratio (low Alb, high immunoglobulin), it indicates monoclonal gammopathy (multiple myeloma & Waldenstrom’s macroglobulinemia)
T or F
T
