1.2.6 Blood Collection and Specimen Consideration (Preanalytical Variables) Flashcards
Age
a. Uncontrollable factors
b. Controllable factors
a
Gender
a. Uncontrollable factors
b. Controllable factors
a
Smoking
a. Uncontrollable factors
b. Controllable factors
b
Underlying condition
a. Uncontrollable factors
b. Controllable factors
a
Diurnal Variation (Increased):
Cortisol
a. AM
b. PM
a
Diurnal Variation (Increased):
Iron
a. AM
b. PM
a
What is the recommended timing of collection for cortisol?
8 AM and 4 PM
Detects cushing syndrome (No changes in the afternoon)
Iron is 30% lower in the __________
Afternoon
Diurnal Variation (Increased):
Growth hormone
a. AM
b. PM
b
Diurnal Variation (Increased):
ACTH
a. AM
b. PM
a
Diurnal Variation (Increased):
PTH
a. AM
b. PM
b
Diurnal Variation (Increased):
Aldosterone
a. AM
b. PM
a
Diurnal Variation (Increased):
ACP
a. AM
b. PM
b
Diurnal Variation (Increased):
TSH
a. AM
b. PM
b
Posture (Increase)
Alb
a. Standing
b. Supine to upright
a and b
Posture (Increase)
Cholesterol
a. Standing
b. Supine to upright
a
Posture (Increase)
Protein and bound substances
a. Standing
b. Supine to upright
b
Posture (Increase)
Calcium
a. Standing
b. Supine to upright
a
Posture (Increase)
Renin
a. Standing
b. Supine to upright
a
Posture (Increase)
Aldosterone
a. Standing
b. Supine to upright
a
Activity (Increased)
TP
a. Ambulatory
b. Exercise
a
Activity (Increased)
Lactate
a. Ambulatory
b. Exercise
b
Activity (Increased)
CK
a. Ambulatory
b. Exercise
a and b
Activity (Increased)
Creatinine
a. Ambulatory
b. Exercise
b
Activity (Increased)
LD, AST, Aldosterone
a. Ambulatory
b. Exercise
b
Catecholamines (Ephinephrine, Nonephinephrin), Cortisol, ACTH, Aldosterone, Prolactine is increased in what factor?
Stress
Diet:
Fasting Plasma glucose
a. REQUIRE fasting
b. BASAL fasting
a
Diet:
OGTT (Oral glucose tolerance test)
a. REQUIRE fasting
b. BASAL fasting
a
Diet:
Triglyceride, Glucose
a. REQUIRE fasting
b. BASAL fasting
a and b
Diet:
Lipid profile
a. REQUIRE fasting
b. BASAL fasting
a
Diet:
Insulin
a. REQUIRE fasting
b. BASAL fasting
a
Diet:
Cholesterol
a. REQUIRE fasting
b. BASAL fasting
b
Diet:
Electrolyte
a. REQUIRE fasting
b. BASAL fasting
b
Cholesterol is Not required for fasting
T or F
T
Required BASAL fast
Diet:
Gastrin
a. REQUIRE fasting
b. BASAL fasting
a
Causes Increase in CO, NH3, and BUN (specifically in Urease method - False increase)
Smoking
IV contamination:
Glucose
a. False increase
b. False decrease
a
IV contamination:
Dilutional effect (BUN - hydration)
a. False increase
b. False decrease
b
IV contamination:
Electrolytes and drug
a. False increase
b. False decrease
a
Hemoconcentration causes False ____________ due to:
- Potassium
- Lactate
- Protein and protein-bound substance
Increase
Most greatly affected when it comes to Hemolysis (False increase)
Intracellular ions: Potassium, Magnesium, Phosphate
Which among the three is the most abundant Cation?
Potassium
Most greatly affected when it comes to Hemolysis (False increase)
Intracellular ions: Potassium, Magnesium, Phosphate, iron
Which among the three is the most abundant Anion?
Phosphate (PO4)
Most greatly affected when it comes to Hemolysis (False increase)
Enzymes: LD, ACP, ALP, AST, ALT, ChE
T or F
T
Slight hemolysis has an significant affect on CK
T or F
F
Not significant
Handling:
ABG, Ammonia, Lactate, ACTH
a. Ice/Chilling
b. Anaerobic
c. X light
a
Handling:
ABG, ammonia, ACP, ionized Calcium
a. Ice/Chilling
b. Anaerobic
c. X light
b
Handling:
Bilirubin, porpyrin, Carotene, Vit A and B12
a. Ice/Chilling
b. Anaerobic
c. X light
c
