3.1.3 Enzymology Intro (MEasurement of Enzyme activity and Other factors that influence enzymatic reactions)

Question 1

IU and Katal is used for Expression of Enzyme Activity

T or F

Answer 1

T

Question 2

Which among is Traditional or Conventional unit?

a. IU
b. Katal

Answer 2

a

Question 3

Which among is SI unit?

a. IU
b. Katal

Answer 3

b

Question 4

Amount of enzyme that catalyzed the reaction or umoL of substance per min

umoL / min

a. IU
b. Katal

Answer 4

a

Question 5

Amount of enzyme that catalyzed the reaction of mol or substrate per sec

mol/sec

a. IU
b. Katal

Answer 5

b

Question 6

Conversion:

1 IU = 0.0167 ukat
1 kat = 6x10^7 IU

T or F

Answer 6

T

Question 7

Convert

32 IU = ______ ukat

then convert it into _______ mkat

Answer 7

0.5344

0.0005344

Solution:

32 x 0.0167
= 0.5344

0.5344 / 1000
= 0.0005344

Question 8

Convert

3 kat = ______IU

Answer 8

1.8 x 10^8

Solution

3 x (6x10^7)
= 1.8 x 10^8

Question 9

Endpoint

a. Fixed time
b. Continuous monitoring

Answer 9

a

Question 10

Kinetic

a. Fixed time
b. Continuous monitoring

Answer 10

b

Question 11

Factors Affecting enzyme reaction includes:

pH
Temperature
Cofactors

T or F

Answer 11

T

Question 12

What is the optimal pH for most enzymes?

a. 7 - 8
b. 4 - 5
c. 9 - 10
d. 6 - 7

Answer 12

a

Question 13

What is the optimal pH for Acid phosphatase (ACP)?

a. 7 - 8
b. 4 - 5
c. 9 - 10
d. 6 - 7

Answer 13

b

Question 14

What is the optimal pH for Alkaline phosphatase (AKP)?

a. 7 - 8
b. 4 - 5
c. 9 - 10
d. 6 - 7

Answer 14

c

Question 15

What is being added to satisfy the pH requirement for enzymes?

a. Buffer
b. Substrate
c. Additional Enzymes

Answer 15

a

Question 16

For each ___C increase in temperature, the rate of the reaction DOUBLE

Answer 16

10

Question 17

Denaturation of Enzymes is usually significant at what C?

a. 40-50
b. 30-40
c. 50-60
d. 60-70

Answer 17

a

Question 18

The endpoint of enzymes when temp is increase

a. Denaturation
b. Lysis
c. Hydrolization
d. None of the above

Answer 18

a

Question 19

A nonprotein entities that must bind to particular enzymes before a reaction ocurrs

a. Enzyme
b. Cofactor
c. Substrate
d. Catalyst

Answer 19

b

Question 20

Types of cofactor that is inorganic

a. Activators
b. Coenzymes

Answer 20

a

Question 21

Types of cofactor that is organic

a. Activators
b. Coenzymes

Answer 21

b

Question 22

Serve as second substrates for enzyme reaction

a. Activators
b. Coenzymes

Answer 22

b

Question 23

May be essential for the reaction or may only enhance the reaction rate

a. Activators
b. Coenzymes

Answer 23

a

Question 24

Includes metallic and non metallic ions

a. Activators
b. Coenzymes

Answer 24

a

Question 25

Called prosthetic groups when bound tightly to the enzyme a. Activators b. Coenzymes

Answer 25

b

Question 26

Mg, Ca, Zn, Cl a. Activators b. Coenzymes

Answer 26

a

Question 27

Mg is needed for more than 300 enzymes T or F

Answer 27

T

Question 28

The chelating agent can be used in Activators in enzyme assay T or F

Answer 28

F No chelating agents

Question 29

NAD(P) / NAD(P)H a. Activators b. Coenzymes

Answer 29

b

Question 30

Pyridoxal phosphate (Vit B6) a. Activators b. Coenzymes

Answer 30

b

Question 31

A prosthetic group that serve in AST/ALT a. Holo Enzyme b. Mg c. Pyridoxal Phosphate

Answer 31

c

Question 32

Binding site: Allosteric site a. Competitive b. Non-Competitive c. Uncompetitive

Answer 32

b

Question 33

Binding site: Active site a. Competitive b. Non-Competitive c. Uncompetitive

Answer 33

a

Question 34

In Competitive, If the inhibitor has a higher concentration than the substrate, then the active site is mostly occupied by inhibitor T or F

Answer 34

T

Question 35

Binding site: Enzyme substrate complex a. Competitive b. Non-Competitive c. Uncompetitive

Answer 35

c

Question 36

Effect of adding substrate: Increase inhibition a. Competitive b. Non-Competitive c. Uncompetitive

Answer 36

c

Question 37

Effect of adding substrate: No affect a. Competitive b. Non-Competitive c. Uncompetitive

Answer 37

b

Question 38

In noncompetitive: if the inhibitor cause a change in the enzymes, it is IRREVERSIBLE and the substrate will never bing T or F

Answer 38

T

Question 39

Effect of adding substrate: Reverse inhibition a. Competitive b. Non-Competitive c. Uncompetitive

Answer 39

a

Question 40

In uncompetitive: The inhibitor can ONLY be bound if there is a substrate attached to the enzyme T or F

Answer 40

T

Question 41

In competitive: Diluting the sample can cause reverse inhibition causing the substrate to be more concentrated T or F

Answer 41

T

Question 42

Macroenzyme is High-molecular-mass forms of the SERUM enzymes that can be bound to Immunoglobulin and non immunoglobulin substance T or F

Answer 42

T Immunoglobulin = Type 1 Nonimmunoglobulin = Type 2

Question 43

Enzymes with macroforms

Answer 43

Macroenzymes

Question 44

Enzymes with macroforms: CK Amylase LD AST ALT ALP LPS GGT Which of the following does not belong?

Answer 44

None