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MTAP 1 Clinical Chemistry > 3.1.3 Enzymology Intro (MEasurement of Enzyme activity and Other factors that influence enzymatic reactions) > Flashcards

3.1.3 Enzymology Intro (MEasurement of Enzyme activity and Other factors that influence enzymatic reactions) Flashcards

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1
Q

IU and Katal is used for Expression of Enzyme Activity

T or F

A

T

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2
Q

Which among is Traditional or Conventional unit?

a. IU
b. Katal

A

a

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3
Q

Which among is SI unit?

a. IU
b. Katal

A

b

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4
Q

Amount of enzyme that catalyzed the reaction or umoL of substance per min

umoL / min

a. IU
b. Katal

A

a

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5
Q

Amount of enzyme that catalyzed the reaction of mol or substrate per sec

mol/sec

a. IU
b. Katal

A

b

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6
Q

Conversion:

1 IU = 0.0167 ukat
1 kat = 6x10^7 IU

T or F

A

T

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7
Q

Convert

32 IU = ______ ukat

then convert it into _______ mkat

A

0.5344

0.0005344

Solution:

32 x 0.0167
= 0.5344

0.5344 / 1000
= 0.0005344

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8
Q

Convert

3 kat = ______IU

A

1.8 x 10^8

Solution

3 x (6x10^7)
= 1.8 x 10^8

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9
Q

Endpoint

a. Fixed time
b. Continuous monitoring

A

a

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10
Q

Kinetic

a. Fixed time
b. Continuous monitoring

A

b

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11
Q

Factors Affecting enzyme reaction includes:

pH
Temperature
Cofactors

T or F

A

T

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12
Q

What is the optimal pH for most enzymes?

a. 7 - 8
b. 4 - 5
c. 9 - 10
d. 6 - 7

A

a

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13
Q

What is the optimal pH for Acid phosphatase (ACP)?

a. 7 - 8
b. 4 - 5
c. 9 - 10
d. 6 - 7

A

b

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14
Q

What is the optimal pH for Alkaline phosphatase (AKP)?

a. 7 - 8
b. 4 - 5
c. 9 - 10
d. 6 - 7

A

c

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15
Q

What is being added to satisfy the pH requirement for enzymes?

a. Buffer
b. Substrate
c. Additional Enzymes

A

a

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16
Q

For each ___C increase in temperature, the rate of the reaction DOUBLE

A

10

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17
Q

Denaturation of Enzymes is usually significant at what C?

a. 40-50
b. 30-40
c. 50-60
d. 60-70

A

a

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18
Q

The endpoint of enzymes when temp is increase

a. Denaturation
b. Lysis
c. Hydrolization
d. None of the above

A

a

19
Q

A nonprotein entities that must bind to particular enzymes before a reaction ocurrs

a. Enzyme
b. Cofactor
c. Substrate
d. Catalyst

A

b

20
Q

Types of cofactor that is inorganic

a. Activators
b. Coenzymes

A

a

21
Q

Types of cofactor that is organic

a. Activators
b. Coenzymes

A

b

22
Q

Serve as second substrates for enzyme reaction

a. Activators
b. Coenzymes

A

b

23
Q

May be essential for the reaction or may only enhance the reaction rate

a. Activators
b. Coenzymes

A

a

24
Q

Includes metallic and non metallic ions

a. Activators
b. Coenzymes

A

a

25
Q

Called prosthetic groups when bound tightly to the enzyme

a. Activators
b. Coenzymes

A

b

26
Q

Mg, Ca, Zn, Cl

a. Activators
b. Coenzymes

A

a

27
Q

Mg is needed for more than 300 enzymes

T or F

A

T

28
Q

The chelating agent can be used in Activators in enzyme assay

T or F

A

F

No chelating agents

29
Q

NAD(P) / NAD(P)H

a. Activators
b. Coenzymes

A

b

30
Q

Pyridoxal phosphate (Vit B6)

a. Activators
b. Coenzymes

A

b

31
Q

A prosthetic group that serve in AST/ALT

a. Holo Enzyme
b. Mg
c. Pyridoxal Phosphate

A

c

32
Q

Binding site:

Allosteric site

a. Competitive
b. Non-Competitive
c. Uncompetitive

A

b

33
Q

Binding site:

Active site

a. Competitive
b. Non-Competitive
c. Uncompetitive

A

a

34
Q

In Competitive, If the inhibitor has a higher concentration than the substrate, then the active site is mostly occupied by inhibitor

T or F

A

T

35
Q

Binding site:

Enzyme substrate complex

a. Competitive
b. Non-Competitive
c. Uncompetitive

A

c

36
Q

Effect of adding substrate:

Increase inhibition

a. Competitive
b. Non-Competitive
c. Uncompetitive

A

c

37
Q

Effect of adding substrate:

No affect

a. Competitive
b. Non-Competitive
c. Uncompetitive

A

b

38
Q

In noncompetitive:

if the inhibitor cause a change in the enzymes, it is IRREVERSIBLE and the substrate will never bing

T or F

A

T

39
Q

Effect of adding substrate:

Reverse inhibition

a. Competitive
b. Non-Competitive
c. Uncompetitive

A

a

40
Q

In uncompetitive:

The inhibitor can ONLY be bound if there is a substrate attached to the enzyme

T or F

A

T

41
Q

In competitive:

Diluting the sample can cause reverse inhibition causing the substrate to be more concentrated

T or F

A

T

42
Q

Macroenzyme is High-molecular-mass forms of the SERUM enzymes that can be bound to Immunoglobulin and non immunoglobulin substance

T or F

A

T

Immunoglobulin = Type 1
Nonimmunoglobulin = Type 2

43
Q

Enzymes with macroforms

A

Macroenzymes

44
Q

Enzymes with macroforms:

CK
Amylase
LD
AST
ALT
ALP
LPS
GGT

Which of the following does not belong?

A

None

MTAP 1 Clinical Chemistry (97 decks)

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