3.1.3 Enzymology Intro (MEasurement of Enzyme activity and Other factors that influence enzymatic reactions) Flashcards
IU and Katal is used for Expression of Enzyme Activity
T or F
T
Which among is Traditional or Conventional unit?
a. IU
b. Katal
a
Which among is SI unit?
a. IU
b. Katal
b
Amount of enzyme that catalyzed the reaction or umoL of substance per min
umoL / min
a. IU
b. Katal
a
Amount of enzyme that catalyzed the reaction of mol or substrate per sec
mol/sec
a. IU
b. Katal
b
Conversion:
1 IU = 0.0167 ukat
1 kat = 6x10^7 IU
T or F
T
Convert
32 IU = ______ ukat
then convert it into _______ mkat
0.5344
0.0005344
Solution:
32 x 0.0167
= 0.5344
0.5344 / 1000
= 0.0005344
Convert
3 kat = ______IU
1.8 x 10^8
Solution
3 x (6x10^7)
= 1.8 x 10^8
Endpoint
a. Fixed time
b. Continuous monitoring
a
Kinetic
a. Fixed time
b. Continuous monitoring
b
Factors Affecting enzyme reaction includes:
pH
Temperature
Cofactors
T or F
T
What is the optimal pH for most enzymes?
a. 7 - 8
b. 4 - 5
c. 9 - 10
d. 6 - 7
a
What is the optimal pH for Acid phosphatase (ACP)?
a. 7 - 8
b. 4 - 5
c. 9 - 10
d. 6 - 7
b
What is the optimal pH for Alkaline phosphatase (AKP)?
a. 7 - 8
b. 4 - 5
c. 9 - 10
d. 6 - 7
c
What is being added to satisfy the pH requirement for enzymes?
a. Buffer
b. Substrate
c. Additional Enzymes
a
For each ___C increase in temperature, the rate of the reaction DOUBLE
10
Denaturation of Enzymes is usually significant at what C?
a. 40-50
b. 30-40
c. 50-60
d. 60-70
a
The endpoint of enzymes when temp is increase
a. Denaturation
b. Lysis
c. Hydrolization
d. None of the above
a
A nonprotein entities that must bind to particular enzymes before a reaction ocurrs
a. Enzyme
b. Cofactor
c. Substrate
d. Catalyst
b
Types of cofactor that is inorganic
a. Activators
b. Coenzymes
a
Types of cofactor that is organic
a. Activators
b. Coenzymes
b
Serve as second substrates for enzyme reaction
a. Activators
b. Coenzymes
b
May be essential for the reaction or may only enhance the reaction rate
a. Activators
b. Coenzymes
a
Includes metallic and non metallic ions
a. Activators
b. Coenzymes
a
Called prosthetic groups when bound tightly to the enzyme
a. Activators
b. Coenzymes
b
Mg, Ca, Zn, Cl
a. Activators
b. Coenzymes
a
Mg is needed for more than 300 enzymes
T or F
T
The chelating agent can be used in Activators in enzyme assay
T or F
F
No chelating agents
NAD(P) / NAD(P)H
a. Activators
b. Coenzymes
b
Pyridoxal phosphate (Vit B6)
a. Activators
b. Coenzymes
b
A prosthetic group that serve in AST/ALT
a. Holo Enzyme
b. Mg
c. Pyridoxal Phosphate
c
Binding site:
Allosteric site
a. Competitive
b. Non-Competitive
c. Uncompetitive
b
Binding site:
Active site
a. Competitive
b. Non-Competitive
c. Uncompetitive
a
In Competitive, If the inhibitor has a higher concentration than the substrate, then the active site is mostly occupied by inhibitor
T or F
T
Binding site:
Enzyme substrate complex
a. Competitive
b. Non-Competitive
c. Uncompetitive
c
Effect of adding substrate:
Increase inhibition
a. Competitive
b. Non-Competitive
c. Uncompetitive
c
Effect of adding substrate:
No affect
a. Competitive
b. Non-Competitive
c. Uncompetitive
b
In noncompetitive:
if the inhibitor cause a change in the enzymes, it is IRREVERSIBLE and the substrate will never bing
T or F
T
Effect of adding substrate:
Reverse inhibition
a. Competitive
b. Non-Competitive
c. Uncompetitive
a
In uncompetitive:
The inhibitor can ONLY be bound if there is a substrate attached to the enzyme
T or F
T
In competitive:
Diluting the sample can cause reverse inhibition causing the substrate to be more concentrated
T or F
T
Macroenzyme is High-molecular-mass forms of the SERUM enzymes that can be bound to Immunoglobulin and non immunoglobulin substance
T or F
T
Immunoglobulin = Type 1
Nonimmunoglobulin = Type 2
Enzymes with macroforms
Macroenzymes
Enzymes with macroforms:
CK
Amylase
LD
AST
ALT
ALP
LPS
GGT
Which of the following does not belong?
None
