2.3.1 Nonprotein Nitrogen Compounds (Urea) Flashcards
Urea is the Lowest concentration in the blood
T or F
F
Highest
Urea comprises of __________ of NPN mass in plasma
a. 45 - 50%
b. 50 - 55%
c. 40 - 45%
d. 35 - 40%
a
Urea is formed in the ______ from CO2 and ammonia
liver
Urea is not associated with the detoxification function of the liver
T or F
F
Associated
Urea is a non toxic form of ammonia
T or F
T
Major excretory product of protein metabolism and other nitrogen-containing chemicals.
Urea
Convert BUN to urea (Formula)
BUN x 2.14
Reference method for Urea
IDMs
Chemical Method for Urea?
a. Jaffe method
b. Phophotungstic Acid method
c. Fearon Method
c
Product for Fearon method when Diacetyl combine with urea?
a. Diacetyl monoxime
b. Formazan
c. Diazine
c
Color of diazine?
Yellow
Enzymatic method for urea:
Colorimetric:
- Nesselrization
- Berthelot
T or F
T
Enzymatic method for urea:
GLDH-Couple enzymatic
T or F
T
Enzymatic method for urea:
Conductimetric is a measurement of the increase in conductivity due to the ___ and CO3^2- ions
a. Urea
b. NH4
c. Uric acid
NH4 (Ammonia)
Berthelot reaction in enzymatic method for urea, the indophenol is color?
Blue
Enzymatic method for Urea:
Glutamate dehydrogenase coupled because the initial reaction is
catalyze by _______
a. Urease
b. Lipase
c. Dehydrogenase
d. Bromase
a
What is the end product for Nesslerization?
a. Indophenol
b. NAD
c. Ammonia and Carbon monoxide
d. Dimercuric ammonium iodide
d
In Nesslerization, the dimercuric ammonium iodide is what color?
Yellow
Enzymatic method for Urea:
If H is the end (NADH) High
absorbance
if D is the end (NAD) decrease absorbance at 340nm
T or F
T
Reference interval for urea
a. 6-20 mg/dl
b. 5-15 mg/dl
c. 7-25 mg/dl
d. 8-20 mg/dl
a
Azotemia:
BUN: Elevated
Creatinine: Normal
Low blood volume, dehydrated, high protein diet, increase protein catabolism
a. Prerenal
b. Renal
c. Postrenal
a
Azotemia:
BUN: Elevated
Creatinine: Elevated
Excretion: Decrease
Glomerulonephritis and renal failure
a. Prerenal
b. Renal
c. Postrenal
b
Azotemia:
BUN: Elevated
Creatinine: Elevated
Excretion: Decrease
Urinary tract obstruction of stone and tumors
Nephrolithiasis, tumors, severe infection
a. Prerenal
b. Renal
c. Postrenal
c
Uremia/Uremic syndrome is always characterized by ___________ of BUN and creatinine.
elevations
Uremia is associated with renal failure and very high plasma urea concentration
T or F
T
