1.3.3 Laboratory Equipment and Supplies (Semi automatic pipettes and Centrifuge)) Flashcards
- Uses suction to draw sample
- polypropylene tip
- piston does not come in contact with the liquid
a. Air displacement
b. Positive displacement
a
- Tips must be rinsed out (Reusable)
- Used for sample in most discrete automated systems
a. Air displacement
b. Positive displacement
b
Operates like a hypodermic syringe
a. Air displacement
b. Positive displacement
b
- No air cushion
- Constant aspiration force for high density or very viscous samples
a. Air displacement
b. Positive displacement
b
Calibration of Semi automatic micropepettors is every?
6 months
Calibration when it comes to gravimetric methods:
Weight of ____________ delivered
Distilled water
Density= 1g/mL
Gravimetric is the most accurate and Spectrophotometric method
T or F
T
Calibration when it comes to the Spectrophotometric method:
Absorbance of ______________
Colored solution
Colored solution includes:
- Potassium dichromate
or
- P-nitrophenol
T or F
T
Use for Body fluid cell counts
a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge
d
Tubes are at fixed angle when rotating
a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge
b
Used to separate layers of different specific gravity
a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge
c
3000 RPM can be only attained in this centrifuge due to friction
a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge
a
In horizontal/swinging bucket centrifuge, the sediment is ______ and tightly pack
Flat
Capable at 7000 RPM due to less friction
a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge
b
