1.3.3 Laboratory Equipment and Supplies (Semi automatic pipettes and Centrifuge))

Question 1

• Uses suction to draw sample
• polypropylene tip
• piston does not come in contact with the liquid

a. Air displacement
b. Positive displacement

Answer 1

a

Question 2

• Tips must be rinsed out (Reusable)
• Used for sample in most discrete automated systems

a. Air displacement
b. Positive displacement

Answer 2

b

Question 3

Operates like a hypodermic syringe

a. Air displacement
b. Positive displacement

Answer 3

b

Question 4

• No air cushion
• Constant aspiration force for high density or very viscous samples

a. Air displacement
b. Positive displacement

Answer 4

b

Question 5

Calibration of Semi automatic micropepettors is every?

Answer 5

6 months

Question 6

Calibration when it comes to gravimetric methods:

Weight of ____________ delivered

Answer 6

Distilled water

Density= 1g/mL

Question 7

Gravimetric is the most accurate and Spectrophotometric method

T or F

Answer 7

T

Question 8

Calibration when it comes to the Spectrophotometric method:

Absorbance of ______________

Answer 8

Colored solution

Question 9

Colored solution includes:

• Potassium dichromate

or

• P-nitrophenol

T or F

Answer 9

T

Question 10

Use for Body fluid cell counts

a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge

Answer 10

d

Question 11

Tubes are at fixed angle when rotating

a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge

Answer 11

b

Question 12

Used to separate layers of different specific gravity

a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge

Answer 12

c

Question 13

3000 RPM can be only attained in this centrifuge due to friction

a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge

Answer 13

a

Question 14

In horizontal/swinging bucket centrifuge, the sediment is ______ and tightly pack

Answer 14

Flat

Question 15

Capable at 7000 RPM due to less friction

a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge

Answer 15

b

Question 16

Causes slanted sendiment

a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge

Answer 16

b

Question 17

capable at 100,000 rpm

a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge

Answer 17

c

Question 18

Gold standard for lipoprotein separation and analysis

a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge

Answer 18

c

Question 19

How does ultracentrifuge attain 100,000rpm?

Answer 19

Refrigeration

Question 20

200-2000 rpm

a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge

Answer 20

d

Question 21

Units used in Centrifugation includes:

• RPM
• RCF (xg)
• Svedberg

T or F

Answer 21

T

Question 22

Svedberg is used in what centri?

a. Horizontal or swinging bucket
b. Fixed-angle or angle-head
c. Ultracentrifuge
d. Cytocentrifuge

Answer 22

c

Question 23

NOTE: (Rpm to RCF)

RCF (g) = 1.118 x 10 ^-5 x r (in cm) x rpm

Question 24

QC:

What is used in parameters to test speed?

Answer 24

Tachometer and timer

Question 25

QC:

Centrifuge is check every?

Answer 25

atleast every 3 months