2.3.2 Nonprotein Nitrogen Compounds (Creatinine)

Question 1

Creatinine comprises ____ of total NPN

Answer 1

5%

Question 2

Creatinine is formed from ______ in the muscles and excreted into plasma at a constant rate

Answer 2

Creatine

Question 3

The concentration of creatinine is inversely related to GFR (Glomerular filtration rate)

T or F

Answer 3

T

Question 4

Chemical Method for Creatinine?

a. Jaffe method
b. Phophotungstic Acid method
c. Fearon Method

Answer 4

a

Question 5

Jaffe method is a colorimetric, endpoint and non-specific

T or F

Answer 5

T

Question 6

Since Jaffe method is non-specific, Other components in plasma may cause False _________ in absorbance

Answer 6

Increase

Question 7

Color of Jaffe method for creatinine?

a. Red
b. Orange
c. Red-Orange

Answer 7

c

Question 8

Techniques to improve specificity of Jaffe reaction are:

• Kinetic: Timed rate reaction
• Use of adsorbents

T or F

Answer 8

T

Question 9

All of the following are adsorbent used to improve the specificity of Jaffe reaction except:

a. Sodium aluminum silicate
b. Magnesium aluminum silicate
c. Calcium aluminum silicate
d. None of the above

Answer 9

C

Question 10

Also known as Lloyd’s adsorbent

a. Sodium aluminum silicate
b. Magnesium aluminum silicate

Answer 10

a

Question 11

Also known as Fuller’s earth adsorbent

a. Sodium aluminum silicate
b. Magnesium aluminum silicate

Answer 11

b

Question 12

Reagent for Jaffe Method?

a. Alkaline picrate
b. Phenol
c. Heparin sulfate-Mn
d. Creatinase

Answer 12

a

Question 13

Enzymatic method for Creatinine:

Creatiniase-POD coupled

T or F

Answer 13

T

Question 14

What is the Product of Creatine + H2O (Creatinase)?

a. Sarcosine
b. H2O2
c. Oxidized chromogen
d. Pyruvate

Answer 14

a

Question 15

Sarcosine + O2 -______-> CH2O + Glycine + H2O2

Answer 15

Sarcosine Oxidase

Question 16

Creatine + ATP -______-> Creatine phosphate + ADP

Answer 16

Creatine Kinase

Question 17

ADP + Phosphenolypyruvate -______-> Pyruvate + ATP

Answer 17

Pyruvate kinase

Question 18

Pyruvate + NADPH -______-> Lactate + NAD

Answer 18

Lactate dehydrogenase

Question 19

Reference interval for Creatinine:

Male: 0.9 - 1.3 mg/dL
Female: 0.6 - 1.1 mg/dL

a. Jaffe
b. Enzymatic

Answer 19

a

Question 20

Reference interval for Creatinine:

Male: 0.6 - 1.1 mg/dL
Female: 0.5 - 0.8 mg/dL

a. Jaffe
b. Enzymatic

Answer 20

b

Question 21

BUN/Creatinine Ratio

Answer 21

10-20:1 normal ratio

Question 22

Creatinine clearance (C) =

Answer 22

(UV/P) x (1.73/A)

Question 23

Reference interval for Creatinine:

Male: 85-125 mL/min
Female: 75-115 mL/min

T or F

Answer 23

T

Question 24

NOTE: Formula for Cockcroft-Gault formula

C = (140 – age x weight in kg)/ (72 𝑥 𝑠𝑒𝑟𝑢𝑚 𝑐𝑟𝑒𝑎𝑡𝑖𝑛𝑖𝑛𝑒(𝑚𝑔/𝑑𝐿))
x 0.85 if F

Question 25

The old version of MDRM has 4 variables while the new ones has 6

T or F

Answer 25

F

Baligtad value

Old: Serum creatinine, Age,
Race, Gender, BUN, and Albumin

New: Serum Creatinine, Age,
Race, and Gender

Question 26

CKD-EPI Formula: Serum
Creatinine, Age, Race, and Gender

T or F

Answer 26

T

Similar to new MDRD formlua

Question 27

4 variables;es are used which is taken into consideration when estimating GFR using Cockcroft-Gault formula?

Answer 27

Age
Weight
Serum Creatinine
Gender

Question 28

If BUN/Creatinine ratio > 20:1 with N creatinine:

Bun is high, the ratio is high

T or F

Answer 28

T

Consistent with Pre-renal azotemia

Question 29

If BUN/Creatinine ratio > 20:1 with ↑ creatinine:

Case of either_____ or post-renal azotemia

Answer 29

renal

Question 30

if BUN/Creatinine ratio < 10:1

Ratio is low, most likely Bun is low

Due to repeated dialysis, liver disease and low protein diet

T or F

Answer 30

T

Question 31

Decreased creatinine clearance is due to impaired _____ function

Answer 31

Renal

Question 32

Decreased creatinine clearance is associated with:

Blood vol.:________
Renal Blood flow:______
GFR:_______

Answer 32

All decrease

Question 33

GFR is dependent on the RBF, and RBF is dependent on BV

T or F

Answer 33

T