2.2.8 Plasma Lipid Analysis (Analytical Method, Lipoprotein Cholesterol)

Question 1

Gold standard for lipoprotein separation and analysis

a. Ultracentrifugation
b. Homogenous assay (Direct)
c. Precipitation

Answer 1

a

Question 2

HDL-C is used in:

a. Ultracentrifugation
b. Homogenous assay (Direct)
c. Precipitation

Answer 2

All of the above

Question 3

Ultracentrifuge Involves adjusting the sample to a density of _______ (potassium bromide) followed by centrifugation at high speed for 24 hours

Answer 3

1.063

Question 4

Immunologic assay involving blockage of non-HDL lipoproteins using an antibody to apo B-100

a. Ultracentrifugation
b. Homogenous assay (Direct)
c. Precipitation

Answer 4

b

All apo B-containing will be removed then left with only HDL

Question 5

Most commonly used for HDL-C determination

a. Ultracentrifugation
b. Homogenous assay (Direct)
c. Precipitation

Answer 5

c

Question 6

Precipitation of apo B-containing lipoproteins (non-HDLs) by polyanion divalent cations (e.g., heparin sulfate-Mn2+, dextran sulfate-Mg2+, sodium phosphotungstate-Mg2+, heparin-Ca2+) followed by cholesterol
determination on the supernatant using the _________________

a. Homologous method
b. POD-coupled method
c. Abell-Kendall assay

Answer 6

b

Question 7

Most commonly used polyanion-divalent cation

a. Dextran sulfate-Mg2+
b. Sodium phosphotungstate-Mg2+,
c. Heparin-Ca2+
d. Heparin sulfate-Mn2+

Answer 7

d

Question 8

CDC reference method includes:

Ultracentrifugation, heparin-Mn2+ precipitation, and cholesterol determination using Abell-Kendall assay

T or F

Answer 8

T

Question 9

Reference Values for HDL-C:

High risk for CHD:________
Desirable: > or equal to 60mg/dL

Answer 9

< 40 mg/dL

Question 10

LDL-C

Uses detergents or other chemicals to block or solubilize non-LDL lipoprotein classes to allow for quantification of LDL

a. Beta quantification
b. Homogenous (Direct)

Answer 10

b

Question 11

LDL-C

Involves ultracentrifugation (to separate VLDL and chylomicrons) and
precipitation (to remove HDL)

a. Beta quantification
b. Homogenous (Direct)

Answer 11

a

Question 12

Formula for TC?

Answer 12

TC = HDL + LDL + VLDL

Question 13

VLDL not routinely measured thus is estimated from TG using the following equations:

▪ Friedewald
− VLDL (mg/dL) = TG / ______
− VLDL (mmol/dL) = TG / 2.175
▪ De Long
− VLDL (mg/dL) = TG / 6.5
− VLDL (mmol/dL) = TG / ______

a. 5, 2.85
b. 5, 2.80
c. 6, 2.85
d. 6, 2.80

Answer 13

a

Question 14

Friedewald calculation is unreliable when TG level is high

T or F

Answer 14

T

Question 15

Friedewald calculation is unreliable when TG level is high
▪ Noticeable error at TG > 200 mg/dL
▪ Error unacceptably high at TG > 400 mg/dL
− Error: falsely __________ LDL

Answer 15

Decreased

Question 16

Reference Values
→ Optimal: < _____ mg/dL
→ Near to Above Optimal: ______mg/dL
→ Borderline High: 130-159 mg/dL
→ High: ______ mg/dL
→ Very High: ≥ ______ mg/d

Answer 16

100

100-129

160-189

190

Question 17

CV: < or equal to 3%
BIAS: < or equal to 3%
Total Error: < or equal to 9%

a. Cholesterol
b. Triglyceride
c. LDL - C
d. HDL - C

Answer 17

a

Question 18

CV: < or equal to 4%
BIAS: < or equal to 5%
Total Error: < or equal to 12%

a. Cholesterol
b. Triglyceride
c. LDL - C
d. HDL - C

Answer 18

d

Question 19

CV: < or equal to 4%
BIAS: < or equal to 4%
Total Error: < or equal to 12%

a. Cholesterol
b. Triglyceride
c. LDL - C
d. HDL - C

Answer 19

c

Question 20

CV: < or equal to 5%
BIAS: < or equal to 5%
Total Error: < or equal to 15%

a. Cholesterol
b. Triglyceride
c. LDL - C
d. HDL - C

Answer 20

b