1.2.2 Blood Collection and Specimen Consideration (Categories of Additives)

Question 1

What is the most preferred specimen in Clinical Chemistry?

Answer 1

Serum

Question 2

If serum cannot be used in CC, what is the alternative for it?

Answer 2

Heparinized plasma

Question 3

Least interference in CC

a. EDTA
b. Citrate
c. Oxalate
d. Heparin

Answer 3

d

Question 4

Color of EDTA for CDC

Answer 4

Lavender

Question 5

Color for EDTA for Crossmatching?

Answer 5

Pink

Question 6

Color of Citrate for Coagulation?

Answer 6

Light Blue

Question 7

Color of Citrate for ESR (Erythrocytic sedimentation rate)

Answer 7

Black

Question 8

Color of Oxalate?

Answer 8

Gray

Question 9

Color of Heparin

Answer 9

Green

Question 10

EDTA, Citrate, and Oxalate is not recommended due to? (MOA)

Answer 10

Removal of Calcium

Question 11

Chelation of Calcium happens which of the following additives:

a. EDTA
b. Citrate
c. Oxalate
d. All of the above

Answer 11

a and b

Question 12

Precipitation of Calcium happens on which of the additive?
a. EDTA
b. Citrate
c. Oxalate
d. All of the above

Answer 12

c

Question 13

EDTA, Citrate, and Oxalate causes a False ________ due to:

• Sodium
• Potassium

Answer 13

Increase

These Additive contains Sodium potassium which cause F increase

Question 14

Which among these additives:EDTA, Citrate, and Oxalate causes false increase in SODIUM?

Answer 14

EDTA (Disodium EDTA)

Question 15

Which among these additives:EDTA, Citrate, and Oxalate causes false increase in Potassium?

Answer 15

Oxalate (Potassium Oxalate)

Question 16

EDTA, Citrate, and Oxalate causes a False ________ due to:

• Calcium

Answer 16

Decrease

Due to removal of Calcium

Question 17

EDTA, Citrate, and Oxalate causes a False ________ due to:

• Mg
• PO4
• Fe

Answer 17

Decrease

Calcium and Magnesium binds to enzymes (Serve as cofactor)

Some of the bind to calcium

Question 18

MOA of Heparin?

Answer 18

Inhibition of thrombin (Thrombin 3)

Question 19

Silica and thrombin are known as _____ activators

Answer 19

Clot

Question 20

Color code for Silica?

Answer 20

Red (Plastic)

Question 21

Color code for Thrombin?

Answer 21

Orange

Question 22

Causes Short clotting time

a. Gel separator
b. Silica
c. Thrombin
d. NaF

Answer 22

b and c

Question 23

Recommended for STAT serum test

a. Gel separator
b. Silica
c. Thrombin
d. NaF

Answer 23

b and c

Question 24

Color for Gel + silica

Answer 24

Gold or Red/black spot

Question 25

Color for Gel + heparin

Answer 25

Light green or Green with Gray spots

Question 26

Which of the following use for SST (Serum separator tube)

a. Red with black spots
b. Light green
c. Green with grey spots
d. Orange

Answer 26

a

Question 27

Which of the following use for PST (Potassium Sensitivity test)

a. Red with black spots
b. Light green
c. Green with grey spots
d. Orange

Answer 27

b and c

Question 28

Color for NaF/Iodoacetate

Answer 28

Grey or Light grey

Question 29

Primarily antiglycolytic agents (MOA)

a. Citrate
b. Oxalate
c. NaF/Iodoacetate
d. Silica

Answer 29

c

Question 30

Gel is not recommended for ___________ since it absorbs it

a. Drug testing
b. Therapeutic Drug monitoring
c. IV drug test
d.Insertion of Drugs via IV

Answer 30

b

Question 31

What are the drugs that are absorbed by the Gel?

a. Tricyclic antidepressants
b. Antiarrhythmic
c. None of the above
d. All of the above

Answer 31

d

Question 32

The drugs that are absorbed by the Gel causes false _________

Answer 32

decrease

Question 33

NaF inhibits __________

Answer 33

Urease

Question 34

MOA for NaF/Iodoacetate is inhibition of __________

Answer 34

Enolase

Question 35

Sodium flouride causes False decrease on what analyte?

Answer 35

BUN