2.2.7 Plasma Lipid Analysis (Analytical Method, Triglyceride) Flashcards
Triglyceride does not require fasting
T or F
F
Required fasting for atleast 12-14 hrs
Chemical method:
Triglyceride + H2O –(Alcoholic KOH)–> glycerol + 3 fatty acids
Glycerol + O2 —(Periodic acid)—> HCHO
T or F
T
Final product of detecting triglyceride?
Formaldehyde
Methods of detecting formaldehyde:
Colorimetric
a. Van Handel-Zilversmith
b. Hantzsch
a
Methods of detecting formaldehyde:
Fluorometric
a. Van Handel-Zilversmith
b. Hantzsch
b
Methods of detecting formaldehyde:
BLue (Orig) or Pink (CDC)
a. Van Handel-Zilversmith
b. Hantzsch
a
Methods of detecting formaldehyde:
Yellow Fluorescence
a. Van Handel-Zilversmith
b. Hantzsch
b
Enzymatic methods:
Triglyceride + H2O –(______)–> glycerol + 3 fatty acids
Glycerol + ATP –(_________)–> Glycerophosphate + ADP
Lipase
Glycerol kinase
Enzymatic Method:
POD-coupled (With color)
Absorbance: _______
NAD/NADH: _______ color
340 nm
No color
What is subsequently detected using POD-coupled reaction?
a. Glycerophosphate
b. Lipase
c. Lipoproteinase
d. Proteinase
a
Reference value for Triglyceride:
Normal:<150 mg/dL
Borderline high: _________
High: ___________
Very high: > or equal to 500mg/dl
150-199 mg/dL
200 - 499 mg/dL
Appearance of plasma (Triglyceride):
Clear: <200 mg/dL
_____: >300 mg/dL
Opaque and Milky: _______
Hazy/Turbid
> 600 mg/dL
As triglyceride increases, the turbidity of the sample also increases
T or F
T
Clinical Significance of Abnormal Results for Triglyceride:
All except 2a,
nephrotic syndrome,
alcoholism,
pancreatitis
a. Hypertriglyceridemia
b. Hypotriglyceridemia
a
Clinical Significance of Abnormal Results for Triglyceride:
Hypothyroidism
Malnutrition
Malabsorption
a. Hypertriglyceridemia
b. Hypotriglyceridemia
b
