Mitochondria and Fluorescent Microscopy

Question 1

Overview and Review

Answer 1

Question 2

What type of Microscopy?

Indicate main feature of mitochondria.

Answer 2

Question 3

Answer 3

Left — Condensed configuration — High ATP production

Question 4

Answer 4

Right — Shelf like cristae — orthodox configuration = low ATP

Question 5

Answer 5

Question 6

Answer 6

Question 7

Answer 7

Question 8

Answer 8

Question 9

Answer 9

Question 10

Answer 10

Question 11

Answer 11

Question 12

Autoradiography

Answer 12

Question 13

What are reporter genes?

Answer 13

Question 14

Proteins and Reporter Genes

Answer 14

Question 15

Answer 15

Question 16

GFP

Answer 16

Question 17

Fluorescence in Embryonic Development

Answer 17

Question 18

Flow Cytometry

Answer 18

Question 19

Flow Cytometry Cont.

Answer 19

Question 20

Even More on Flow Cytometry

Answer 20

Question 21

Immunofluorescence and Microscopy

Answer 21

Question 22

Staining in Immunofluorescence

Answer 22

Question 23

What type of treatment to tissue?

Answer 23

Immunofluorescence

Question 24

Indirect Immunofluorescnece

Answer 24

Question 25

Confocal Microscopy

Answer 25

Question 26

Immunohistochemistry

Answer 26

Question 27

Immunohistochemistry Cont.

Answer 27

Question 28

What technique was used?

Answer 28

Immunohistochemsitry

Question 29

Basic Immunohistochemsitry

Answer 29

Question 30

CISH

Answer 30

Question 31

Tissue Treatment?

Answer 31

Question 32

CISH Continued

Answer 32

Question 33

CISH Pt. 500000

Answer 33

Question 34

Histochemical Stains

Answer 34

Question 35

Enzyme Histochemistry

Answer 35

Question 36

Common Histochemical Stains and Locations

Answer 36

Question 37

Laser Capture Microdissection

Answer 37

Question 38

Laser Capture Pt. 2

Answer 38

Question 39

Slide Scanning

Answer 39

Question 40

Common Scenario in Microscopic Analysis

Answer 40