Genetic Variation

Question 1

How many Haploid BP in the Human Genome

Answer 1

Question 2

What is the current best estimate of number of genes in the human genome?

5000

10,000

15,000

30,000

100,000

Answer 2

30,000

Question 3

How does Phenotypic Variation Arise?

Answer 3

Question 4

Exome

Answer 4

sum of all the exons.

Question 5

Biggest sources of variation

Answer 5

DNA Replication Errors and Meiosis.

Question 6

DNA Replication Errors

Answer 6

DNA Replication Errors is the source of variation that Darwin spoke of

Question 7

On average how many polymorphic differences would yo find if you compared the genomes of two randomly chosen humans?

1 Million

1000

10,000

100,000

10 Million

Answer 7

1 Million (1 Million/23 Chromosomes)

Question 8

Which will give you better polymorphism screens? DNA polymorphisms screens or Protein Polymorphism screens?

Answer 8

DNA Polymorphism Screens

Question 9

Interim Summary: Types of DNA Polymorphisms

Answer 9

Question 10

Which of these is the most automatable of the polymorphisms?

Answer 10

SNPs are Binary and that has revolutionized medicine because we can use binary computer code (1’s and 0’s).

Question 11

How many base pairs differentiates human beings from one another?

How many polymorphisms?

Answer 11

Question 12

RFLP Detection

Answer 12

Result from DNA sequence differences that occur at restriction sites in the human genome. The locations of these sites are found by hybridizing restriction fragments with cloned probes.

Question 13

VNTR polymorphisms are detected on the basis of differences in

Number of G Nucleotides

Secondary Structure

Number of Histones

Number of Promoter Sequences

Molecular Weight

Answer 13

Molecular Weight (Because one will have more repeats than the other we can separate them based on the molecular weights)

Question 14

STRP or VNTR

Answer 14

STRP – Microsattellite Repeats

VNTR – Minisatellite Repeats

Tandem repeats consisting of short segments of DNA (microsatellites) or somewhat longer segments (minisattellites whose length can be14 to 500 bps) that are repeated over and over in tandem.

Question 15

CNV

Answer 15

CNV represents differences in Larger Segments of DNA (bp’s > 1000 to 2 million bp)

Question 16

SNP’s

Answer 16

Question 17

How Can Do we Detect Tandem Repeat Polymorphisms

Answer 17

Question 18

Function of PCR

Answer 18

Question 19

Explain Next Gen Sequencing

Answer 19

Question 20

Defining Variability in Individual Genomes

Answer 20

Question 21

Answer 21

All of the above

Question 22

Explain Snip Chips

Answer 22

Question 23

Visualizing SNP CHIP

Answer 23

Question 24

What are causes of Genetic Variation in Populations

Answer 24

Question 25

Summary of Key Points

Answer 25