Introduction to Microscopy Flashcards

1
Q
A

Blue binds to negatively charged nucleic acids

Pale signifies, euchromatin that theres a lot of expression going on

S is heterochromatic nucleus with small cytoplasm — relatively inactive.

H and E stain

DNA/RNA is blue

Proteins/Amino acids are pink

Hepatocytes

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2
Q
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Artifacts

large lipid droplets

All the components of the cell get pushed off to the periphery. Nucleus, golgi, gets pushed off to the side.

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3
Q
A

Artifacts

small lipid droplets

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4
Q
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2-8

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5
Q
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Answer #3 : 12-18

You are looking at the inside of a vessel and the vessel wall.

Some cells look like they don’t have a nucleus because the cut is such that the nucleus is not included.

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6
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7
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8
Q
A

PAS is electric purple

H&E is a dull purple

Tall columnar cells

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9
Q

What is Metachromasia

A
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10
Q

Increasing Magnification also…

A
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11
Q

Can you increase magnification without increasing resolution?

A

Yes, digitally

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12
Q

What is TEM?

A
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13
Q

Light Microscope vs. TEM

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14
Q

What is an SEM

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15
Q

What type of microscopy?

A

Scanning Electron Microscope

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16
Q

What type of microscopy and what is it scanning

A

Intestinal Epithelium on an SEM

17
Q
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18
Q

Lipid Bilayer

A
19
Q
A

O2 and H2O and Steroids

Charged will have difficult time getting through

Just a little water can pass through (without aquaporins)

Amino acids, glucose, Chloride, none of them can pass through.

20
Q

Lipids in the bilayer

A
21
Q

Proteins in the Lipid Bilayer

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22
Q

How can we attach proteins to the membrane?

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23
Q

6 Functions/Types of Membrane Proteins

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24
Q

Example of Structural Protein

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25
Q

Example of Enzymes in the membrane

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26
Q

Examples of Linkers in the Membrane

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27
Q

Examples of Recpetors

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28
Q

Example of protein channels in the membrane

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29
Q

Example of Protein Pumps in the Membrane

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30
Q

Movement of Molecules through the membrane

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31
Q

Facilitated Diffusion vs. Active Diffusion

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32
Q
A

E — peripheral proteins are only attached to membrane via charged interactions.

Ionic detergents disrupt the membrane.

33
Q

Carbohydrates in the lipid bilayer

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34
Q

Blood, Carbohydrates, and the membrane

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35
Q

Lipid Rafts

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36
Q
A