Introduction to Microscopy Flashcards
Blue binds to negatively charged nucleic acids
Pale signifies, euchromatin that theres a lot of expression going on
S is heterochromatic nucleus with small cytoplasm — relatively inactive.
H and E stain
DNA/RNA is blue
Proteins/Amino acids are pink
Hepatocytes
Artifacts
large lipid droplets
All the components of the cell get pushed off to the periphery. Nucleus, golgi, gets pushed off to the side.
Artifacts
small lipid droplets
2-8
Answer #3 : 12-18
You are looking at the inside of a vessel and the vessel wall.
Some cells look like they don’t have a nucleus because the cut is such that the nucleus is not included.
PAS is electric purple
H&E is a dull purple
Tall columnar cells
What is Metachromasia
Increasing Magnification also…
Can you increase magnification without increasing resolution?
Yes, digitally
What is TEM?
Light Microscope vs. TEM
What is an SEM
What type of microscopy?
Scanning Electron Microscope