Intro to Microscopy Flashcards

1
Q

Describe how a light microscope works?

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2
Q

Knurled Head Locking Screw

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3
Q

The Specimen Holder/Stage

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4
Q

Light Intensity Control Knob

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5
Q

Abbe Condenser

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6
Q

Revolving Nose Pieces and Objective Lenses

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7
Q

Widefield Eyepiece

Interpupillary Scale

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8
Q

Course Focus and Fine Focus Knob

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9
Q

Knurled Diopeter Ring

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10
Q

Knobs Controlling the Mechanical Stage

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11
Q

Identify Basic Features of Cells and Tissues

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12
Q

Cytoplasm vs. Cytosol

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13
Q

Identify the Tissue, The Microscopy, the outlined green, and the blue arrows

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14
Q

Approximate the number of cells.

Identify the Blue Arrows?

Green Outline?

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15
Q

Identify the green brackets

Identify the yellow Arrows

What type of microscopy?

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16
Q

What are the 4 major steps of slide preparation?

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17
Q

Explain the Process of Tissue Fixation

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18
Q

Explain Chemical Fixation

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19
Q

Explain the process of Embedding

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20
Q

Explain the process of sectionig

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21
Q

Explain the Process of Staining

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22
Q

Hematoxylin vs. Eosin

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23
Q

What type of stain is this?

What type of Tissue?

Nucleus vs. Proteins in staining

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24
Q

What type of staining is this?

Explain Cytoplasmic Basophillia.

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25
Q

What type of stain?

What are the green arrows?

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26
Q

What type of stain?

What comprises the outlined the region and why?

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27
Q

What type of stain?

Why the color?

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28
Q

What type of stain?

Identify heterochromatin and Euchromatin.

Red Arrow

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29
Q

Explain Periodic and Acid Schiff

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30
Q

What type of stain?

What gets stained?

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31
Q

Identify the clear protions of these stains

Describe what they are generally and point to them specifically.

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32
Q

Identify The black Arrow?

The Blue Arrow?

Describe the pink space between.

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33
Q

Shrinkage and Artifacts

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34
Q

What type of Stain?

Green Arrows?

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35
Q

What type of stain?

Identify the Blue and Black Arrows.

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