Intro to Microscopy Flashcards by Omar Saqr

Q

Describe how a light microscope works?

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Q

Knurled Head Locking Screw

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Q

The Specimen Holder/Stage

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Q

Light Intensity Control Knob

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Q

Abbe Condenser

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Q

Revolving Nose Pieces and Objective Lenses

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Q

Widefield Eyepiece

Interpupillary Scale

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Q

Course Focus and Fine Focus Knob

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Q

Knurled Diopeter Ring

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Q

Knobs Controlling the Mechanical Stage

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Q

Identify Basic Features of Cells and Tissues

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Q

Cytoplasm vs. Cytosol

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Q

Identify the Tissue, The Microscopy, the outlined green, and the blue arrows

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Q

Approximate the number of cells.

Identify the Blue Arrows?

Green Outline?

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Q

Identify the green brackets

Identify the yellow Arrows

What type of microscopy?

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Q

What are the 4 major steps of slide preparation?

A

Q

Explain the Process of Tissue Fixation

A

Q

Explain Chemical Fixation

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Q

Explain the process of Embedding

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Q

Explain the process of sectionig

A

Q

Explain the Process of Staining

A

Q

Hematoxylin vs. Eosin

A

Q

What type of stain is this?

What type of Tissue?

Nucleus vs. Proteins in staining

A

Q

What type of staining is this?

Explain Cytoplasmic Basophillia.

A

Q

What type of stain?

What are the green arrows?

A

Q

What type of stain?

What comprises the outlined the region and why?

A

Q

What type of stain?

Why the color?

A

Q

What type of stain?

Identify heterochromatin and Euchromatin.

Red Arrow

A

Q

Explain Periodic and Acid Schiff

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Q

What type of stain?

What gets stained?

A

Q

Identify the clear protions of these stains

Describe what they are generally and point to them specifically.

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Q

Identify The black Arrow?

The Blue Arrow?

Describe the pink space between.

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Q

Shrinkage and Artifacts

A

Q

What type of Stain?

Green Arrows?

A

Q

What type of stain?

Identify the Blue and Black Arrows.

A