CILS Molecular Techniques Pt. II Flashcards
True or False?
If a researcher wants to determine if a genes is expressed in cells in a tissue section, the quesiont can be fully answered using either immunohistochemisitry or in in situ hybridization?
False — You need to know whether not the protein is present because the mRNA could be present but proteins not made
True or False?
Infections of virus and bacteria increase risk for cancer?
True
The RB protein acts as a brake stopping uncontrolled progression?
True
RB protein is both a co-repressor and a tumor supressor?
True
Physiological when RB protein interact with E2F protein and it is pathological when RB interacts with E7?
True
Derangements of the retinoblastoma gene product contribute to disease of only the ocular system, where the result is a childhood eye cancer?
False — you can have problems with kidney.
Summary of Primary Paper on Papillomavirus and E7
Summary of Primary Paper on Papillomavirus and E7 Continued
No band is seen in the lysate called RBL and the reason for this is:
Those cells were not incubated with anti-RB antibody
Those cells do not express Rb due to Rb gene mutations.
This cells do not express RB due to an over expressed transcription repressor protein
None of the above
Those cells do not express Rb due to Rb gene mutations. — tumors don’t have RB proteins because RB is a tumor suppressor.
Goal of coimmuno-precipitation studies commonly make the use of an antibody agains only one of the multiple proteins present in a sample, the goal usually being?
To detect whether protein-protiene interactions are present.
To demonstrate that the protein is recognized by the antibody.
To detect whether protein-protiene interactions are present.
Middle lane corresponds with a cell lysate that was incubated with an anti-T antibody and this lane functions in the experiment as a:
Negative Control
Positive Control
Both of the Above
None of the Above
Negative Control
The reason they could see informative bands with this technique is because of?
Pigment deposition using an enzyme conjugated antibody against an anti-RB antibody.
Pigment deposition using an enzyme conjugated directly to an anti-RB antibody
pigment deposition using an enzyme conjugated directly to an anti-E7 & antibody
None of the above
None of the above — these were radio labeled
RB prevents E2F from acting as an activator and enhancing transcription.
Summary of Additional Paper on the Expression Profile of p53
Tetramer
