5.6 - Monoclonal Antibodies Flashcards
What monoclonal antibodies are The uses of monoclonal antibodies in medicine The use of antibodies in the ELISA test
What are monoclonal antibodies?
Antibodies from a single clone of plasma cells
What does ‘mono’ mean?
Single
What does ‘clonal’ mean?
Clone
Are monoclonal antibodies identical or different?
Identical
What are some uses of monoclonal antibodies?
Diagnosis
What is an ELISA test?
Enzyme-Linked ImmunoSorbant Assay
What does an ELISA test detect?
Presence and quantity of protein
What are the two types of ELISA test?
Direct and indirect
How many antibodies does a direct ELISA test use?
One
How many antibodies does an indirect ELISA test use?
Two
What is the first step in an indirect ELISA test?
Add sample to well plate
What is the second step in an indirect ELISA test?
Add antibody specific to target protein
What is the third step in an indirect ELISA test?
Wash the well
What is the fourth step in an indirect ELISA test?
Add second antibody with enzyme
What is the fifth step in an indirect ELISA test?
Wash the well again
What is the sixth step in an indirect ELISA test?
Add substrate solution
What does the intensity of the color indicate?
Quantity of protein present
