21.6 - Gel Electrophoresis Flashcards
What gel electrophoresis is Running electrophoresis on DNA samples Visualising and analysing results from gel electrophoresis
1
Q
What is gel electrophoresis?
A
Technique to separate molecules by size using electric current
2
Q
How do you set up gel electrophoresis?
A
Insert gel tray
3
Q
How do you load samples in gel electrophoresis?
A
Mix DNA with loading dye
4
Q
Why do DNA molecules move during electrophoresis?
A
Negative charge due to phosphate groups
5
Q
What happens during electrophoresis?
A
Voltage applied
6
Q
How does size-based separation occur?
A
Gel mesh slows larger fragments more
7
Q
How do you visualise results from gel electrophoresis?
A
Switch off voltage
