2.2 - DNA Replication

Question 1

What is semi-conservative replication?

Answer 1

DNA replication where each new molecule has one original and one new strand

Question 2

What enzyme unwinds the DNA double helix?

Answer 2

DNA helicase

Question 3

What enzyme joins free nucleotides together during DNA replication?

Answer 3

DNA polymerase

Question 4

In what direction does DNA polymerase join nucleotides?

Answer 4

5’ to 3’

Question 5

What type of bonds are formed between nucleotides?

Answer 5

Phosphodiester bonds

Question 6

What was the Meselson-Stahl experiment designed to determine?

Answer 6

The method of DNA replication

Question 7

What are the two proposed models of DNA replication?

Answer 7

Conservative and semi-conservative

Question 8

What isotopes of nitrogen were used in the Meselson-Stahl experiment?

Answer 8

14N and 15N

Question 9

What was the result of the Meselson-Stahl experiment?

Answer 9

DNA replication is semi-conservative

Question 10

What happens during the first replication in the Meselson-Stahl experiment?

Answer 10

One heavy and one light strand in each DNA molecule

Question 11

What happens during the second replication in the Meselson-Stahl experiment?

Answer 11

Half heavy-light