1.11 Factors Affecting Enzyme Action Flashcards
What happens when an enzyme is denatured The effects of temperature and pH on enzyme-catalysed reactions The effects of enzyme and substrate concentration on enzyme-catalysed reactions
1
Q
What is enzyme denaturation?
A
- change in the enzyme’s tertiary structure
- prevents substrate binding
2
Q
What causes enzyme denaturation?
A
Changes in temperature or pH
3
Q
How does temperature affect enzyme activity?
A
Increases activity to an optimum but post the optimum can lead to denaturing
4
Q
How does pH affect enzyme activity?
A
- in acidic conditions can denature enzymes
- at the optimum pH the enzyme works its fastest
- in alkaline conditions the enzyme can denature
5
Q
How does substrate concentration affect enzyme activity?
A
Increases activity until saturation
6
Q
How does enzyme concentration affect enzyme activity?
A
Increases activity until substrate becomes limiting
7
Q
What happens at an enzyme’s optimum temperature?
A
The enzyme works fastest
8
Q
What happens at an enzyme’s optimum pH?
A
The enzyme works fastest
9
Q
What happens when an enzyme is denatured?
A
The active site changes shape
