21.7 - Genetic Fingerprinting Flashcards
What genetic fingerprinting is The process of creating a genetic fingerprint Applications of genetic fingerprinting
1
Q
What is genetic fingerprinting?
A
Technique to identify unique DNA patterns
2
Q
What are VNTRs?
A
Variable number tandem repeats
3
Q
What are key features of VNTRs?
A
Present in eukaryotes
4
Q
What are STRs?
A
Short tandem repeats
5
Q
What are the steps of creating a genetic fingerprint?
A
DNA extraction
6
Q
How is DNA extracted?
A
From tissue sample
7
Q
How is DNA digested?
A
Restriction enzymes cut DNA near VNTR sequences
8
Q
How are fragments separated?
A
Gel electrophoresis
9
Q
What is hybridisation?
A
Radioactive or fluorescent probes bind to VNTR sequences
10
Q
How is the fingerprint developed?
A
Probes revealed
11
Q
What are applications of genetic fingerprinting?
A
Paternity testing
12
Q
What are limitations of genetic fingerprinting?
A
Environmental contamination
