2.3.1 - Transcription Flashcards
Where Does Transcription Take Place?
Nucleus.
What Does Transcription Produce?
mRNA.
Describe The Process Of Transcription
(6 Points)
~ A segment of DNA unwinds, breaking hydrogen bonds between complementary base pairs, exposing the gene to be transcribed.
~ RNA polymerase catalyses the formation of a complementary mRNA strand from the exposed DNA gene.
~ Free activated RNA nucleotides pair with complementary bases on the exposed DNA strand via hydrogen bonds.
~ RNA polymerase bonds the sugar-phosphate groups of the RNA nucleotides, forming the mRNA’s sugar-phosphate backbone.
~ Once the mRNA strand is complete, it detaches from the DNA, which reforms its double-stranded structure.
~ The mRNA molecule exits the nucleus through a pore in the nuclear envelope.
What Is The Antisense / Template Strand?
Strand of DNA molecule used to produce the mRNA molecule.
What Is The Sense / Coding Strand?
Other strand of DNA molecule.
Which Direction Does RNA Polymerase Move Along The Antisense Strand?
3’ to 5’ direction.
Which Direction Does The mRNA Molecule Grow In?
5’ to 3’ direction.