TM 3 (Pre-transfusion) Flashcards
Blood sample must be retained
7days
Donors must be tested for ___ before labeling as Rh-
weak D
RNA tests on blood
HCV
HIV
Diseases tested for
HepB HepB HIV 1 & 2 HTLV 1 & 2 RPR West Nile
Can do forward typing only if____
<4mo
ABO discrepancy with lack of expected Ag
Transfusion/transplant
Leukemia
ABO discrepancy with unexpected Ag
Acquired B
Transfution/transplant
Auto-agglutination
ABO discrepancy with lack of expected Ab
Immunodeficiency
Neonate
Transplant/transfuse
ABO subgroup
ABO discrepancy with unexpected Ab
Anti-A1 Ab to reagent rouleax cold auto-/allo- transplant/transfuse
NAT testing
HCV, HIV, West Nile
Window period for HCV with NAT
10-30d
Window period for HIV with NAT
10d
Shelf-like for isovolemic hemodilution
8 hr at RT
24 hr refrigerated
shelf life for intraoperative blood salvage
4 hr at RT
24 hr refrigerated
shelf life for post-op blood salvage
6hr at RT
HIV testing
Screen: anti-HIV EIA and NAT
Confirm: WB or IFA
HCV testing
Screen: Anti-HCV and NAT
Confirm: RIBA
HBV testing
Screen: HBsAg and Anti-Hbc
Confirm: neutralization for HBsAg
HTLV testing
ANti-HTLV EIA
Syphilis testing
Screen: EIA, RPR
Confirm: FTA
Chagas
Screen: EIA
Confirm: RIPA
indications for plasmapheresis
TTP/HUS Waldenstrom MG GB goodpasture hypercholesterolemia
contraindications to plts
TTP, HIT, ITP, DIC, post-transfusion purpura
standard dose of plts
1 random donor unit per 10kg body weight
good dose for most products in neonate
10-15ml/kg
how long can plts go without agitation
24hr
leukocyte reduction indicated to prevent
febrile transfusion
HLA alloimmunization
CMV transmission
people who need irradiated units
congenital Tcell defects (DiGeorge, SCID, Wiskott-Aldrich) Stem cell transplant Fludarabine intrauterine or premature first-degree HLA matched relatives
uses for cryo
fibrinogen deficiency tx vWD tx uremic thrombocytopathy factor XIII def tx hemophilia A (factor VIII)
