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Clinical Pathology > Microbiology 8 (bacterial biochemical tests) > Flashcards

Microbiology 8 (bacterial biochemical tests) Flashcards

1
Q

Catalase test

A

+ Staph and Micrococcus

- Strep and Enterococcus

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2
Q

Furazolidone susceptibility test

A

Staph susceptible

Micrococci resistant

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3
Q

Coagulase test

A

+ S. aureus

- all other staph and micrococcus

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4
Q

Novobiocin susceptibility

A

Staph. saphrophyticus resistant

Staph. epidermidis susceptible

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5
Q

Bacitracin and TMP-SMX for Beta-hemolytic strep

A 
Group B (Strep. agalaciae) resistant to both
Group A (Strep. pyogenes) resistant to only TMP-SMX
All other Strep. susceptible to both
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6
Q

6.5% NaCl tolerance

A

Enterococcus tolerant
Group D strep not
(both gamma-hemolytic)

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7
Q

CAMP test

A

Beta-hemolytic activity of S. aureus is enhanced by Group B strep (S. agalactiae) or Listeria

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8
Q

PYR test

A

In B-hemolytic strep: S. pyogenes +, S. agalactiae -)

In G-hemolytic strep: Enterococci +, Group D-)

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9
Q

Bile esculin test

A

Enterococci and Group D strep +

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10
Q

Bile solubility test

A

Strep. pneumonia are soluble in bile

Viridans strep insoluble

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11
Q

Optochin test

A

(P-disk)

inhibits Strep. pneumoniae

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12
Q

Cytochrome oxidase

A 
All Enterobacteriaceae (-)
(+) Vibrio, Aeromonas, Pseudomonas, Burkholderia, Plesiomonas
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13
Q

Indole test

A

E.coli (+)

P. vulgaris (+)

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14
Q

Nitrate reduction

A

Enterobacteriaceae (+)

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15
Q

Urease

A

(+) Proteus

(+) Klebsiella

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16
Q

String test

A

Vibrio cholerae

17
Q

Staph streak test

A

Haemophilus grows as satellites around S. aureus

18
Q

CHO utilization

A

glucose, maltose, sucrose, lactose for Neisseria

19
Q

CHO utilization of N. gonorrhea

A

glucose only

20
Q

CHO utilization of N. meningitidis

A

maltose (and glucose)

21
Q

CHO utilitzation of N. lactamica

A

lactose (and glucose/maltose)

22
Q

CHO utilization of M. catarrhalis

A

all negative

23
Q

Organisms requiring X factor only

A

H. ducreyi

24
Q

Organisms requiring V factor only

A

H. parainfluenza
H. parahaemolyticus
H. aphrophilus

25
Q

Organisms requiring X and V factors

A

H. influenza
H. haemolyticus
H. aegyptius

26
Q

DNAse

A

M. catarrhalis is only G- that is (+)

S. aureus is (+)

27
Q

Reverse CAMP

A

B-hemolysis of Strep. agalactiae (Group B) is enhanced by Clostridium perfringens

27
Q

Hippurate

A

S. agalactiae (group B) +

C. jejuni +

28
Q

Phenylalanine deaminase +

A

Proteus
Morganella
Providencia

29
Q

Hydrogen sulfide +

A

Salmonella
Edwardsiella
Citrobacter
Proteus

Clinical Pathology (59 decks)

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