Microbiology 8 (bacterial biochemical tests) Flashcards
Catalase test
+ Staph and Micrococcus
- Strep and Enterococcus
Furazolidone susceptibility test
Staph susceptible
Micrococci resistant
Coagulase test
+ S. aureus
- all other staph and micrococcus
Novobiocin susceptibility
Staph. saphrophyticus resistant
Staph. epidermidis susceptible
Bacitracin and TMP-SMX for Beta-hemolytic strep
Group B (Strep. agalaciae) resistant to both Group A (Strep. pyogenes) resistant to only TMP-SMX All other Strep. susceptible to both
6.5% NaCl tolerance
Enterococcus tolerant
Group D strep not
(both gamma-hemolytic)
CAMP test
Beta-hemolytic activity of S. aureus is enhanced by Group B strep (S. agalactiae) or Listeria
PYR test
In B-hemolytic strep: S. pyogenes +, S. agalactiae -)
In G-hemolytic strep: Enterococci +, Group D-)
Bile esculin test
Enterococci and Group D strep +
Bile solubility test
Strep. pneumonia are soluble in bile
Viridans strep insoluble
Optochin test
(P-disk)
inhibits Strep. pneumoniae
Cytochrome oxidase
All Enterobacteriaceae (-) (+) Vibrio, Aeromonas, Pseudomonas, Burkholderia, Plesiomonas
Indole test
E.coli (+)
P. vulgaris (+)
Nitrate reduction
Enterobacteriaceae (+)
Urease
(+) Proteus
(+) Klebsiella
String test
Vibrio cholerae
Staph streak test
Haemophilus grows as satellites around S. aureus
CHO utilization
glucose, maltose, sucrose, lactose for Neisseria
CHO utilization of N. gonorrhea
glucose only
CHO utilization of N. meningitidis
maltose (and glucose)
CHO utilitzation of N. lactamica
lactose (and glucose/maltose)
CHO utilization of M. catarrhalis
all negative
Organisms requiring X factor only
H. ducreyi
Organisms requiring V factor only
H. parainfluenza
H. parahaemolyticus
H. aphrophilus
Organisms requiring X and V factors
H. influenza
H. haemolyticus
H. aegyptius
DNAse
M. catarrhalis is only G- that is (+)
S. aureus is (+)
Reverse CAMP
B-hemolysis of Strep. agalactiae (Group B) is enhanced by Clostridium perfringens
Hippurate
S. agalactiae (group B) +
C. jejuni +
Phenylalanine deaminase +
Proteus
Morganella
Providencia
Hydrogen sulfide +
Salmonella
Edwardsiella
Citrobacter
Proteus