Microbiology 6 (bacterial media and stains) Flashcards

1
Q

Kinyoun stain

A

acid fast without heat

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2
Q

Fite (modified acid fast)

A
weaker acid (H2SO4 instead of HCl)
Nocardia and M.leprae
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3
Q

AFB+ organisms

A
mycobacteria, 
nocardia, legionella, rhodococcus
cryptosporidium, microsporidium, isospora
sarcocystis
saccharomyces
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4
Q

BCYE

A

Legionella

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5
Q

Thayer-Martin

A

Neisseria

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6
Q

MacConkey

A

G- rods

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7
Q

Red on MacConkey

A

Eschericia
Klebsiella
Enterobacter

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8
Q

Pink on MacConkey

A

Citrobacter
Providencia
Serratia
Hafnia

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9
Q

Colorless on MacConkey

A

Proteus
Edwardsiella
Salmonella
Shigella

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10
Q

EMB agar

A

G- rods

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11
Q

Green/black/purple on EMB

A

Eschericia, Klebsiella, Enterobacter, Serratia, Hafnia, Citrobacter, Providencia

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12
Q

Colorless on EMB

A

Proteus, Edwardsiella, Salmonella, Shigella

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13
Q

Salmonella on SS agar

A

colorless with black center

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14
Q

Shigella on SS agar

A

colorless

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15
Q

Salmonella and shigella on HE agar

A
salmonella = green with black center
shigella = green
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16
Q

Mannitol salt agar (MSA)

A

isolate S. aureus

17
Q

Listeria grows at _____, motility at ____

A

grows 37

motility 25

17
Q

TCBS agar

A

Vibrio cholerae is sucrose positive (yellow)

V. parahemolyticus is sucrose neg

18
Q

Bordet-gengeau agar

A

Bordatella

19
Q

Barber-Stoenner-Kelley medium

A

Borrelia

20
Q

Media for mycobacteria

A

Lowenstein Jensen

Middlebrook 7H11