Microbiology 6 (bacterial media and stains)

Question 1

Kinyoun stain

Answer 1

acid fast without heat

Question 2

Fite (modified acid fast)

Answer 2

weaker acid (H2SO4 instead of HCl)
Nocardia and M.leprae

Question 3

AFB+ organisms

Answer 3

mycobacteria, 
nocardia, legionella, rhodococcus
cryptosporidium, microsporidium, isospora
sarcocystis
saccharomyces

Question 4

BCYE

Answer 4

Legionella

Question 5

Thayer-Martin

Answer 5

Neisseria

Question 6

MacConkey

Answer 6

G- rods

Question 7

Red on MacConkey

Answer 7

Eschericia
Klebsiella
Enterobacter

Question 8

Pink on MacConkey

Answer 8

Citrobacter
Providencia
Serratia
Hafnia

Question 9

Colorless on MacConkey

Answer 9

Proteus
Edwardsiella
Salmonella
Shigella

Question 10

EMB agar

Answer 10

G- rods

Question 11

Green/black/purple on EMB

Answer 11

Eschericia, Klebsiella, Enterobacter, Serratia, Hafnia, Citrobacter, Providencia

Question 12

Colorless on EMB

Answer 12

Proteus, Edwardsiella, Salmonella, Shigella

Question 13

Salmonella on SS agar

Answer 13

colorless with black center

Question 14

Shigella on SS agar

Answer 14

colorless

Question 15

Salmonella and shigella on HE agar

Answer 15

salmonella = green with black center
shigella = green

Question 16

Mannitol salt agar (MSA)

Answer 16

isolate S. aureus

Question 17

Listeria grows at _____, motility at ____

Answer 17

grows 37

motility 25

Question 17

TCBS agar

Answer 17

Vibrio cholerae is sucrose positive (yellow)

V. parahemolyticus is sucrose neg

Question 18

Bordet-gengeau agar

Answer 18

Bordatella

Question 19

Barber-Stoenner-Kelley medium

Answer 19

Borrelia

Question 20

Media for mycobacteria

Answer 20

Lowenstein Jensen

Middlebrook 7H11