Chemistry 4 (Proteins) Flashcards
recommended method for total protein
Biuret technique
copper salts turn purple colon
absorbance at 540nm
proteins absorb UV light at
210 and 280 nm
T1/2 albumin
17d
fastest migrating protein on SPEP
prealbumin (transthyretin)
2 functions of prealbumin
binds T3 and T4
complexes with Vitamin A
prealbumin is the amyloid precursor in
senile cardiac amyloidosis
T1/2 of prealbumin is
48hr
true increase in prealbumin seen in
chronic alcoholic
corticosteroid use
predominant alpha 1 band
alpha1-antitrypsin
minor component of alpha band but highly increased in acute inflammation
alpha1-acid glycoprotein
large protease inhibitor which is not lost in nephrotic syndrome
alpha2-macroglobulin
3 components of alpha 2 band
alpha2-macroglobulin
ceruloplasmin
haptoglobin
3 components of beta band
transferrin
fibrinogen
complement (C3)
transferrin increased in
iron deficiency
pregnancy
estrogen
transferrin in CSF
double peak from asialated transferrin (Tau)
Carbohydrate deficient transferrin is a marker for
EtOH use
fibrinogen may be seen in serum in
heparin treatmtnte, dysfibrinogenemia, APL, liver dz, Vit K def
protein besides Ig in gamma region
CRP
dz assd with selective loss of albumin
minimal change
band that is not decreased in nephrotic syndrome
alpha2 (alpha2 macroglobulin)
SPEP in acute inflammation
increased alpha 1 and alpha2
decreased albumin
eventual polyclonal increase in Ig
beta-gamma bridging seen in
cirrhosis (due to increased IgA)
monoclonal protein seen in
myeloma, waldenstrom (LPL), CLL/SLL
biclona peak can be see in what gammopathy
IgA due to monomers and dimers
____ assay very sensitive to myeloma recurrence
free light chain assay
Hyperviscosity syndrome when viscosity >___
3cp
measure viscosity when Ig___
IgM>4
IgG or A >6
Characteristics of CSF electrophoresis
increased prealbumin
double B (transferrin)
dimmer albumin
Characteristics of glomerular proteinuria
very increased albumin
increast AAT
increased transferrin
characteristics of tubular proteinuria
increased alpha 2, beta, and light chains
procedure to detect cryoglobulins
draw, clot, and centrifuge at 37
store at 4 for 3d then centrifuge at 4
type 1 cryoglobulinemia
monoclonal (MM or waldenstrom)
Type 2 cryoglobulinemia
monoclonal IgM (rheumatoid factor) with polyclonal IgG
type 3 cryoglobulinemia
2 polyclonal Ig’s
most common cuase of mixed cryoglob
HCV
sx of cryoglob
leukocytoclastic vasculitis (palpable purpura)
arthralgias, organomegaly
lymphadenopathy, anemia
sensorineural defects, glomerulonephritis
other lab abnormality in cryoglobulinemia
hypocomplementemia
most common renal finding in cryoglob
MPGN type II (dense deposit dz)
