Immunology 2

Question 1

HLA class II structure

Answer 1

2 chains (alpha and beta) similar to Ig light chains with transmembrane domain

Question 2

Clues to Bcell/immunoglobulin defect

Answer 2

bacterial infections of respiratory tract

recurrent/persistant Giardiasis

Question 3

Clues to Tcell defect

Answer 3

viral and fungal infections

Question 4

Phagocytic defects

Answer 4

staph infections

Question 5

Terminal complement defect

Answer 5

encapsulated bacterial (Strep. pneumo, Neisseria)

Question 6

Microlymphocytotoxicity assay

Answer 6

To detect HLA class I antigens or antibodies
HLA antisera are incubated with Pt lymphocytes with excess complement and dye (color is + reaction)

Question 7

mixed lymphocyte culture detects

Answer 7

HLA class II diferences

Question 8

HLA class ___ are 2nd importance only to ABO compatibility in solid organ transplants

Answer 8

Class II

Question 9

mixed lymphocyte culture method

Answer 9

dissimilarities in HLA cause proliferation of lymphs

Question 10

Matching for transplant involes at least

Answer 10

A, B, DR

Question 11

what is mediator of hyperacute rejection

Answer 11

HLA abs

Question 12

transplant crossmatch done by

Answer 12

incubating donor lymphos with recipient serum + complement

Question 13

HLA matching is not as important in which organs

Answer 13

heart and lung

Question 14

Antibodies to protein antigens requres ____ cells

Answer 14

Tcells and B cells

Question 15

antibodies to carbohydrate antigens requires ____ cells

Answer 15

B cells only

Question 16

Most common immunoglobulin defect

Answer 16

IgA deficiency

Question 17

Conditions assd with increased IgE

Answer 17

parasites,
Churg-Strauss,
Job syndrome,
IgE myeloma,
Hodgkin dz

Question 18

RAST

Answer 18

allergen specific IgE measurement (best for inhaled antigens)
not a screening test

Question 19

Delayed type hypersensitivity reaction uses ___ cellls

Answer 19

T cells

Question 20

Mitogens used in T cell proliferation assays

Answer 20

phytohemagglutinin, concanavalin A

Question 21

severe infection reported in isolated NK cell deficiency

Answer 21

herpes virus

Question 22

how to tes for NK cell function

Answer 22

chromium release assay

Question 23

nitroblue tetrazolium test

Answer 23

<10% cells purple in chronic granulomatous dz (assd with McCloud phenotype/Kx negative)

Question 24

Screening test for classical complement system

Answer 24

CH50 (% of Ig-coated sheep red cells lysed by patient serum)

Question 25

Quantitative deficiences of which complement components will decrease CH50

Answer 25

C1-9

Question 26

decreased levels of C3 reflect

Answer 26

primary C3 deficiency

activation of alternate pathway

Question 27

C4 and C1q levels look at

Answer 27

classical pathway

Question 28

which immune defects don’t appear until around 6 months

Answer 28

B cell

Question 29

Bruton agammaglobulinemia genetics

Answer 29

Atk gene on ChX

Question 30

Testing in Bruton agamma

Answer 30

very decreased IgG, decreased circulating B cells, no geminal centers, no plasma cells

Question 31

other conditions assd with Brutons agamma

Answer 31

leukemia/lymphoma, autoimmune dz