TM 2 (Blood collection and processing) Flashcards
Temp requirements
<37.5 (99.5)
Pulse
50-100
BP
<180/100
H/H
allogeneic >12.5/38%
autologous >11/33%
low volume unit definition
300-404mL
normal volume unit
450 +/- 45mL
Storage temp for RBCs
1-6C
Storage temp if to be used for components
20-24C
Needs to be refrigerated in how much time if to be used for RBCs
within 8 hr
Pheresis platelet requirements
2 days between
no more than 2x/wk
no more than 24x/yr
If ? RBCs present, crossmatch required
> 2ml
Ab screen on neonate done on
mother or baby
Red cell storage times defined by
75% survival of transfused RBCs after 24hr
Preservatives with 21d storage
CPD, CP2D
Preservaties with 35d storage
CPDA-1
Preservatives with 42d storage
AS-1,-3,-5
Additive solutions with mannitol
AS-1, AS-5
storage requirements for frozen rbcs
10yr at -65
24hr after thaw
storage for washed RBCs
24hr at 1-6C
storage for plts
5d at 20-24
storage for granulocytes
24hr at 20-24
storage for FFP
1 yr at -18
7yr at -65
24hr after thaw at 1-6C
storage for cryo
1yr at -18
6 hr after thaw at 20-24
HCT and volume of pRBCs
250-350
Hct <80%
WBCs, RBCs in leukoreduced RBC
<5x10^6 WBC in 95%
retain 85% of RBC
plts in single plt unit
> 5.5x10^10
pH >6.2 in 90%
50mL volume
~80mg fibrinogen
plts in leukoreduced single plt
> 5.5x10^10 in 75%
pH > 6.2 in 90%
<8.3x10^5 WBC in 95%
Plts in apheresis plt
> 3x10^11 in 90%
specifics of leukoreduced apheresis plt
> 3x10^11 and plt > 6.2 in 95%
<5x10^6 WBC
cryo specifics
Factor XIII >80IU
FIbrinogen >150
specifics of granulocyte concentrates
> 1x10^10 in 75%
specifics of whole blood
450-550 mL
RBC 200mL
Plasma 250Ml
63 or 70ml anticoagulant
Iron in RBCs
200-250mg in 1 unit
1mg iron/mL
shipping temp for RBCs
1-10C
definition of leukoreduced
<5x10^6 WBC (5mil) in 95%
or 8.3x10^5 in a platelet concentrate
radiation dose
25Gy to center
15Gy to all
maximun storage after irradiation
28d
FIbrinogen and volume of FFP
200-250mL
400mg fibrinogen
2 units FFP increases factor levels by
20-30%
cryopoor plasma has significantly less
vWF