Hematology 1 Flashcards by Casey Gooden

Q

Type of Hgb not measured by cyanohemoglobin method

A

sulfhemoglobin

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Q

absorbance of cyanohemoglobin method

A

540nm

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Q

(by manual methods) MCV =

A

(Hctx100)/RBC

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Q

MCHC =

A

Hb/Hct

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Q

Automated methods directly measure

A

RBC, MCV, RDW

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Q

RBC size in fL

A

36-360

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Q

(automated methods) HCT =

A

MCVxRBC

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Q

Plt size in fL

A

2-20

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Q

Absolute retic count =

A

%retic x RBC

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Q

Correction factors for RPI

A

0 at 45
0 at 30
0 at 15

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Q

Corrected Retic count (CRC) =

A

% retic x (Hct/45)

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Q

retic production index (RPI) =

A

CRC x (1/correction factor)

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Q

Hbs detected by solubility (dithionate) test

A

SS, SA, SC, SD, C harlem

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Q

Hbs detected by sickling (metabisulfite) test

A

SS, SA, SC, SD, C harlem

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Q

Hbs that run with A2 on alkaline

A

C,E, O, C harlem

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Q

Hbs that run with S on alkaline

A

D, G, Lepore

Q

Hbs that run with A on alkaline

A

M

Q

Hbs that run with A on acid

A

A2, D, G, E, M

Q

Hbs that run with S on acid

A

C harlem

Q

Location of HbH and Barts on alkaline

A

closest to anode (+)

Q

Pancellular pattern on acid elution

A

most hereditary persistence of fetal hemoglobin

Q

Normal % of A2

A

<3%

Q

Thalassemic indices

A

Increased RBC, decreased MVC

Q

HbA2 increased in which thalassemia

A

Beta

Q

5 normal peaks on HPLC

A

degretation products
Glycated A1c
degredation products
HbA
Hb A2

Q

decreased LAP score

A

CML, PNH, MDS, congenital hypophasphatasia, neonatal septicemia

Q

increased LAP score

A

leukemoid, non-CML myeloproliferative, steroids, preganancy

Q

Heterocellular pattern on acid elution

A

fetomaternal hemorrhage, thalassemia

Q

CD43 underexpressed in

A

Wiskott-Aldrich syndrome