Hematology 1 Flashcards
Type of Hgb not measured by cyanohemoglobin method
sulfhemoglobin
absorbance of cyanohemoglobin method
540nm
(by manual methods) MCV =
(Hctx100)/RBC
MCHC =
Hb/Hct
Automated methods directly measure
RBC, MCV, RDW
RBC size in fL
36-360
(automated methods) HCT =
MCVxRBC
Plt size in fL
2-20
Absolute retic count =
%retic x RBC
Correction factors for RPI
- 0 at 45
- 0 at 30
- 0 at 15
Corrected Retic count (CRC) =
% retic x (Hct/45)
retic production index (RPI) =
CRC x (1/correction factor)
Hbs detected by solubility (dithionate) test
SS, SA, SC, SD, C harlem
Hbs detected by sickling (metabisulfite) test
SS, SA, SC, SD, C harlem
Hbs that run with A2 on alkaline
C,E, O, C harlem
Hbs that run with S on alkaline
D, G, Lepore
Hbs that run with A on alkaline
M
Hbs that run with A on acid
A2, D, G, E, M
Hbs that run with S on acid
C harlem
Location of HbH and Barts on alkaline
closest to anode (+)
Pancellular pattern on acid elution
most hereditary persistence of fetal hemoglobin
Normal % of A2
<3%
Thalassemic indices
Increased RBC, decreased MVC
HbA2 increased in which thalassemia
Beta
