TEZT END OF YEAR 1

Question 1

what does the ELISA test stand for

Answer 1

enzyme-linked immunosorbent assay

Question 2

what is the ELISA test

Answer 2

an immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples. Some examples include: diagnosis of HIV infection, pregnancy tests, and measurement of cytokines or soluble receptors in cell supernatant or serum.

Question 3

what is the first step of the ELISA test

Answer 3

HIV antigen bound to bottom of vessel

Question 4

what is the second step of the ELISA test

Answer 4

Blood plasma sample is added, antibodies specific to HIV antigen will attach, ones that are not will not attach

Question 5

What is the fourth step of ELISA test

Answer 5

Substrate added (enzyme reacts with substrate, forming a coloured product)

Question 6

what’s the third step of the ELISA test

Answer 6

secondary antibody is added

Question 7

magnification equation

Answer 7

Magnification = image size / actual size. Actual size =