Cell Fractionation

Question 1

What is cell fractionation

Answer 1

Process where cells are broke. Up and different organelles they contain are spread out

Question 2

What is the first step of cell fractionation

Answer 2

Tissue to be studied is cut into small pieces (minced) and placed into,a cold,isotonic and buffered solution

Question 3

What is the second step of cell fractionation

Answer 3

These are then ground into smaller pieces using a homogeniser (sophisticated liquidizer) releasing the organelles from the cel

Question 4

What is the third step of cell fractionation

Answer 4

The homogenate is filtered to remove any complete cells and large debris i.e. cell wall membrane

Question 5

What is the fourth step of cell fractionation

Answer 5

A suspension of homogenate is then placed in a test tube and then centrifuged-the faster the tube is spun ,the greater the force generated

Question 6

What is the fifth step of fractionation

Answer 6

At slower speeds the larger fragments collect at the bottom of the tube and smaller ones remain at the top suspended in a liquid called the supernatant liquid

Question 7

What is the 6th step of cell fragmentation

Answer 7

These larger fragments (sediment pellets) are then removed and the supernatant remaining is re - spun at a faster speed (more force) and some of the smaller fragments collect at the bottom forming a new pellet

Question 8

What is the seventh and last step of cell fragmentation

Answer 8

By continuing this way very smaller and smaller fragments will be recovered

Question 9

What is the isolation medium

Answer 9

-place tissue sample into solution to prootect the organelles before breaking the cells open

Question 10

Why are conditions cold in cell fragmentation

Answer 10

To reduce enzyme activity that might break down the organelles

Question 11

What is the order from large to small organelles

Answer 11

Nucleus,mitochondria,lysosomes,ribosomes