Microscopy ID Flashcards
What are the steps in a Gram stain preparation?
- Mount the specimen on the slide
- Stain with crystal violet
- Use iodine as a mordant so the color isn’t washed out
- EtOH/Acetone wash (Gram + will stay violet)
- Counterstain with safari (Gram - will stain pink)
What are the best specimens for a direct exam?
- Sputum
- Urine
- CSF
- Vaginal Smear
What are Acid Fast stains used for?
They are used for bacteria with cell walls of long chains of fatty acids
What are examples of bacteria that Acid Fast stains?
- Mycobacteria
- Nocardia
- Cyclospora
- Cryptosporidia
What are the 4 fungal stains?
Lactophenol Cotton Blue
GMS
PAS
Mucicarmine
Lactophenol Cotton Blue stains….
Histoplasma
GMS stains…
Yeast cells and hyphae
Pneumocystis carinii
Aspergillus
PAS stains…
Yeast cells and hyphae
Aspergillus
Mucicarmine stains…
Cryptococcus
What are the parasite stains?
Wright Giemsa
Wet Mounts
Trichrome
Modified Acid Fast
What is the Warthin-Starry stain?
Silver stain for bacteria that stains spirochetes and cat scratch disease
Wright Giemsa stains…
Histoplasma
Leishmania
Trypanosomas
Malaria
Wet Mounts stain…
Giardia
Trichrome stains…
Giardia
What are the fluorescence stains?
Auramine-Rhodamine
Calcofluor White
Flourescein Conjugated Antibodies
Staphylococcus aureus
Neisseria meningitides in CSF
E. coli in blood
Actinomyces
Campylobacter in jejunum
Nocardia
