History/Basic Concept & Drosophila development Flashcards
Specification State
Received instructions, but doesn’t have to follow, can still be influenced by the environment where it can change its fate if the environment changes (really more on potential than fate so it’s less committed)
Determination State
Received instructions but can’t change fate has to follow instructions. It also can’t be influenced by environment so potential & fate are equal therefore its more committed
Testing Specification
They removed a cell from its embryo (an eye cell) & grew it in a neutral media with no new stimulus, when they put the eye cell into a neutral media it was not specified if it didn’t receive signals to become an eye & express eye gene (blastula)
When testing specification what resulted if the eye cell was specified?
If it was specified it received signal to become an eye & express eye gene (gastrula)
Testing determination
They put an eye cell in a non-neutral environment so that it does have different stimulus & also challenge the cell in different environments
What happened when testing the determination when the cell is not determined?
If the cell is not determined, then its influence by the environment where if you put an eye cell into muscle environment it becomes a muscle (gastrula)
What happened when testing the determination when the cell is determined?
If the cell is determined, then it’s not influenced by the environment where if your put an eye cell (Or neurula) into a muscle environment it still stays as an eye cell
Development Commitment
- Specification (less committed) (blastula to gastrula)
- Determination (more committed) (gastrula to neurula)
- differentiation (neurula to embryo)
Induction
Is cell to cell signaling, where one cell induces another
What are the different inductive interactions??
- Diffsuion
- Direct Contact
- Gap Junction
Diffusion
An induction interaction where one cell has a receptor
Direct Contact
An inductive interaction where both cells have receptors
Gap Junction
An inductive interaction where both cells are connected together
The inducer in an inductive interaction
Makes the signal in an inductive interaction
The responder in an inductive interaction
In an inductive interaction the responder gets induced only if its competent (has a receptor or signaling pathway)
How can a cell lose its competence over time?
By differential gene expression
Morphogen
Molecule secreted by an inducer (specifies by concentration gradient & cells respond to different concentration)