Gene Transcription & RNA modification part 3 Flashcards
What are the 3 types of proteins needed for basal transcription at the core promoter?
- RNA polymerase 2
- General transcription factors (5 GTF)
- Mediator
Basal transcription
Baseline expression which has many genes & doesn’t have regulatory proteins
_____ are always need for RNA polymerase 2 to initiate transcription of genes
5 GTF
Describe the first 3 steps to form a open complex & start transcription?
- TFIID binds to TATA box
- TFIIB binds to TFIID
- TFIIB binds TFIIF & RNA polymerase
What are the last 3 steps to form a open complex & start transcription?
- TFIIE & TFIIH bind to RNA polymerase 2 to form a preinitiation complex (closed complex)(PIC)
- TFIIH acts as a helicase to form an open complex by unwinding the DNA & it also acts as as kinase
- TFIIB, TFIIE, & TFIIH released to form an open complex
Kinase
Adds phosphate & it then phosphorylates the tail of RNA polymerase 2
TFIID
Is a complex of proteins that includes the TATA binding protein (TBP) & severally TBP associated factors (TAFs)
Mediator
Medicates interactions between RNA polymerase 2 & regulatory transcription factors that bind to enhancers or silencers
Transcriptional termination of __________ occurs after the 3’ end of the transcript is cleaved near the polyadenylation signal sequence
RNA polymerase
What are the 2 models in termination
- Allosteric Model
2. Torpedo Model
Allosteric Model
After transcribing the polyadenylation signal sequence, RNA polymerase 2 is destabilized & falls off from the DNA (cause a change in its shape)
Torpedo Model
An exonuclease binds to the 5’ end of the RNA that is still transcribed & degrades it in the 5’ to 3’ direction & polymerase falls off
Describe termination process
- RNA polymerase 2 transcribed a gene past the polyadenylation signal sequence
- The RNA is cleaved just past the polyadenylation signal sequence
- Either the allosteric model is occurs or the torpedo model occurs to end termination
___________ occurs after transcription where rRNA remove spacers, tRNA adds CCA & mRNA cap, spliced, & polyadenylated
RNA modifications
________ formed in the nucleolus & it is transcribed, processed & bound to ribosomes where its modification makes mRNA short
rRNA
Exon
Contain coding sequences that are translated into proteins & found in mature mRNA
Introns
Found between exons & contain coding sequences that aren’t translated into proteins
What happens with tRNA?
Makes mRNA go from long to short where both ends are digested, CCA to 3’ end & modifies nucleotides
The production of tRNA molecules require what two things?
- Exonuclease
2. Endonuclease
Exonuclease (RNaseD)
Cleaves a covalent bond between two nucleotides at one end of a strand & digest a strand