96. Main features of the boar’s ejaculate. Method of boar semen collection and preservation Flashcards
Boar sperm Parameters?
Boar sperm parameters
Volume of ejaculate 200ml
Number of sperm per ejaculate 60Mrd
Sperm concentration in AI dose ~30,000,000/ml
Number of sperm per AI dose 2-4Mrd
Number of AI doses per ejaculate 20
Frequency of sperm recovery 2 times a week maximum
Boar semen collection?
Boar Semen Collection
Artificial vagina
- Already not used
- Inability to observe penis and secretions
Gloved-hand method
- Easy observation of penis and seminal fluid is possible
- First phase is not collected
- Seminal plasma is filtered out
Electroejaculation
• Used exceptionally only under general anaesthesia
Boar training
- First on female
- Then on phantom treat with board scent
- Can be dangerous
US-bag method
- Disposable, aseptic
- Built in filter
- Dilution in the bag
- Equipped with dispensing connector
Primary observation of boar semen?
Primary observation
• Macroscopic
o Volume, colour, smell
• Microscopic
o Concentration, motility, morphology
Quality parameters for semen?
Quality parameters for semen
Volume (without gel mass) 100-300ml
Motility at time of collection Minimum 70%
Concentration Minimum 108 sperm cells/ml
Abnormal sperm cells <20%
- One AI dose > 2x109 motile sperm cells and > 80ml
- Head abnormalities are more important that that of the tail
Normal boars ejaculate?
Normal boars ejaculate
Characteristic Normal value Limit value
Ejaculate volume (ml) 100-500 50
Total sperm number (billion) 10-100 10
Progressive motility (%) 70-90 70
Abnormal sperm heads (%) 2-5 10
Proximal cytoplasmic droplets (%) 1-5 5
Acrosome abnormalities (%) 1-2 5
Abnormal midpieces (%) 2-5 5
Single-bent sperm tails (%) 1-5 25
Steps of liquid sperm conservation?
Steps of liquid sperm conservation
Origin ejaculate
|
Filtration
|
Sperm evaluation
|
Determination of dilution
|
Dilution
|
Manufacture of sperm dose (80-100ml)
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Transport (16°c)
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Storage (16°c)
