35. Clinical features of pregnancy proteins in cattle Flashcards
1
Q
Pregnancy associated glycoproteins(PAG) ?
A
Pregnancy associated glycoprotein PAG) subtypes
- Aspartate proteinase family members, proteolytic enzymes
- Cell specific expression
- Unknown function
2
Q
bPAG-1?
A
bPAG-1
- Genitally younger cells produce
- Only binucleate cells produce
- After day 25 of pregnancy proteins cross basement membrane and show up in maternal circulation
- Higher plasma concentration in crossed animals
3
Q
bPAG-2?
A
bPAG-2
- Genitally older cells produce
- Mono and binucleate cells produce
- Cells are located mostly at the foetomaternal border of the placenta
- PAG 2 production starts earlier and lasts longer in the placenta
4
Q
On farm PAG measurement?
A
On farm PAG measurement
- Rapid visual PAG test
- Sandwich type ELISA
5
Q
Pregnancy proteins in cattle days?
A
Cow
- Day 18-20 (starts production, increasing until end of gestation)
- Continuously produced during pregnancy
- Migrate and invade the endometrial epithelium.
- Advantage
o Specific
• Disadvantage
o Accurate from day 30
o Long half-life after calving
o Long half-life after embryonic mortality!!
o Not as accurate as for US
6
Q
Conclusion of pregnancy proteins?
A
Conclusion
• Most tests are only accurate after day 30!!
7
Q
Future?
A
Future:
• Early pregnancy factors
o USA: Early Conceptus Factor Test (cow-side test) available now.
o Low specificity → high number of F+ diagnoses!
