Lec 7-3 - Enterobacteriaceae Flashcards
entero are gram blank
negative rods (motile or non motile)
entero do not form blank
spores
entero grow better with blank but can grow without it
air
entero are killed by temperatures above blank
60 degrees C
entero are not killed by
freeze/thaw
• E. coli • Klebsiella pneumonia • Enterobacter • Citrobacter • Salmonella • Shigella • Serratia • Proteus • Providencia • Morganella • Yersinia sp., including Y. pestis • Noted are all blank
enterobacteriaceae
• Campylobacter • Vibrio • Aeromonas • Plesiomonas • Helicobacter • pseudomonas are all blank
not entero
lactose fermenting gram negative rods are called blank
coliforms
increased coliform means increased blank of things like water
fecal contamination
selective indicator agar for enterics
macconkey agar
on macconkey plate, lactose fermenters turn blank and non lactose fermenters turn blank
pink, white
most abundant microbe in the colon capable of growing aerobically
e coli
surface marker antigens of e coli
o, h, k
antigen of e colithat has somatic antigens, is stable to boiling, and is a repeating sugar on LPS
O
antigen of e coli that has flagellar antigens coming out from all sides (halo)
H
antigen of e coli that are capsular antigens which some e coli has but some dont
K
two diarrheagenic e coli we need to know
ETEC, STEC (EHEC)
most common cause of travelers diarrhea in third world countries
etec
etec strains can produce these toxins
LT, ST
LT is heat blank
labile
ST is heat blank
stable
LT stimulates blank
cAMP
ST stimulates blank
cGMP
LT and ST are both enterotoxins and both pour out blank
watery fluid
the e coli more common in developed countries
EHEC/STEC, bloody diarrhea
transmission from blank can occur with EHEC/STEC
waterparks/swimming pools
most notorius EHEC strain that is more virulent
strain 0157
only EHEC blank enter the body but the blank do not
toxins, bacteria
stx1 of ehec works by cleaving a particular adenine residue from 16S rRNA which halts blank
protein synthesis
stx2 is like stx1 but it also blocks blank not just halts it
protein synthesis
blank is worse than blank
stx2, stx1
most common cause of uti in outpatients
e coli
second most common cause of uti in outpatients
proteus
infection confined to the urinary bladder
cystitis
more severe version of cystitis that extends to the ureters and kidney
pylonephritis
all e coli express this type of pili
1
invasive enteric infectious organisms that dont just stay in the lumen and go into the mucus and are invasive
salmonella, shigella
shigella are most likely to produce blank which is a terrible diarrhea that is chronic and is the butts equivalent of dry heaving
shigella
detecting salmonella and shigella on macconkey agar would show blank
white colonies
new sensitive agar for detecting salmonella
XLD agar
salmonella produces blank colonies on XLD
black (easy to spot)
blank inocolum pathogens are usually in contaminated water or food held at higher temperatures like salmonella… secondary cases are rare
high
blank inoculum pathogens can be transmitted by bodily contact, swimming pools, ice, can have secondary cases like cruise ships
low
most common cause of salmonella food poisoning like in eggs, chicken, pet reptiles
salmonella enterica
salmonella naming
o E.g. Salmonella enterica Serotype Enteritidis
o Or Salmonella enterica Serotype Saintpaul
