Lec 3-4 - Staph Flashcards
three important staphs
staph aureus, staph epidermidis, staph saprophyticus
staph size
.7-1.2 microns
s aureus are usually blank color and blank hemolysis
off white or yellow, beta
s epidermidis are usually blank color colonies
opaque white
staph is not blank so it can grow on bunch of agars
fastidious
do staph form spores
no
do staph move
no
nutrients created by staph
facultative anaerobe
staph is catalase blank
positive usually
coagulase positive means blank
pathogenic staph
s aureus and intermedius is coagulase blank
positive
s epidermidis is coagulase blank
negative
peptidoglycan makes up this much of cell wall weight, teichoic acid makes up this much, protein a makes up this much
50, 40, 5
s aureus is resistant to blank but susceptible to blank
lysozyme, lysostaphin
teichoic acid binds to blank in the host
fibronectin
protein a binds to blank of antibodies
Fc
s saprophyticus are usually in blank
genitourinary
staph is associated with these parts of the mouth but not the gingival crevice
saliva, teeth
staph and strep are usually the cause of blank lesions
root caries
main carriers of s aureus
noses of hospital personnel, vagina during periods
s epidermidis causes blank
uti hospital acquired (opportunistic), endocarditis, biofilms
s saprophyticus causes blank
uti community acquired
staph usually causes these infections
cutaneous, serious diseases, pneumonia, septicemia, food poisoning, toxic shock
staph toxins are dangerous and can cause food poisoning and are superantigens and heat stable up to 100 degrees C for several minutes and can be used as a blank
bio warfare agent
s epidermidis is blank resistant to methicillin
80 percent
biofilm formation by s epidermidis is induced by blank and encoded by blank
stress, ica operon
biofilm is often formed on blank
prosthetic devices
penicilllin and methicillin are blank antibioitcs interfere with cell wall formation by messing with PBP
beta lactam
vancomycin antibiotics are blank antibiotics and interfere with cell wall formation by binding to D Ala D Ala residues
glycopeptide
vancomycin is used to treat blank infections
mrsa
cyclic lipopeptide that binds to bacterial cell membrane causes rapid depolarization
daptomycin
beta lactamase resistance is due to an enzyme that destroys the beta lactam ring via the encoding of this gene
blaZ
intrinsic methiciillin resistance is from the staphylococcal cassette chromosome blank
mec (SCCmec)
main way to tell apart HA MRSA and CA MRSA
HA MRSA doesnt have PVL (leukotoxin) but CA does
HA infections are usually more blank
invasive
CA infections are usually more blank
necrotizing
CA infections are genetically different than HA and are susceptible to blank antibioitics
non beta lactam… such as tetracycline, sulfa drugs, vancomycin
this is for s aureus screening
CHROMagar of nasal/rectal samples
