Infectious Diseases - Bacteria Overview Flashcards
What type of organisms are bacteria?
Prokaryotes - no nucleus or organelles
How do bacteria reproduce?
Through binary fission
Bacteria contain ____
Plasmids
What do plasmids do?
Confer antibiotic resistance and other traits
What are the 4 steps for Gram staining?
- Crystal violet dye
- Iodine traps dye
- Alcohol decolorizes
- Safranin (counterstrain)
Gram (+) bacteria stains?
PURPLE
Purple bacteria indicates?
Gram (+)
Gram (-) bacteria stains?
RED
Red bacteria indicates?
Gram (-)
Gram (+) bacteria has a ____ peptidoglycan wall
Thick
Gram (-) bacteria has a ____ peptidoglycan wall
Thin
What causes Gram (-) bacteria to lose the crystal violet dye?
Lipid A endotoxin and lipopolysaccharides on surface
What are the gram stain exceptions?
Gram variable bacteria
Acid Fast Bacilli
Cocci
Spherical shaped
Dipolococci
2 spheres
Streptococci
Chain of spheres
Staphylococci
Cluster of spheres
Bacilli
Rod shaped
Diplobacili
2 rods
Streptobacilli
Chain of rods
Bacilli can contain?
Spores
Bacilli can also be what shaped?
Club shaped rods
Spirochete
Helical shaped
Spirilla
S shaped
Vibrio
Comma shaped
Filamentous
Thread-like
Pleomorphic
No particular shape
Coccobacillus
Ovoid shape
Attributes of a Gram Stain test?
Cheap
Rapidly detects presence and general type of bacteria
Attributes of a Culture test?
SLOW
Aerobic and Anaerobic cultures
Attributes of a Molecular Technique?
Expensive
Sensitive and Specific test!
Attributes of Serology?
Detects antibody positivity for organisms that do not culture well
What are the 4 steps to Gram Staining?
- Crystal Violet Dye
- Iodine traps dye
- Alcohol decolorizes
- Safranin (counterstrain)