properties and formation of dental plaque :) Flashcards

1
Q

what is plaque

A

biofilm mixture of different species of bacteria embedded in a matrix of polymers

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2
Q

what is a microbiome

A

all the bacteria in the body

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3
Q

what is an oral microbiome

A

all the bacteria in the oral cavity

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4
Q

where can micro biome be found in babies not born

A

in amniotic fluid

rupture to membrane can mean that bacteria can enter the placenta

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5
Q

post natal acquisition of oral flora

A

normal delivery

c section

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6
Q

what does the micro biome reflect from normal delivery

A

vaginal tract microbiota

e.g. lactobacillus

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7
Q

what does the micro biome reflect through c section

A

skin microbiiota

e.g. staphylococcus

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8
Q

what does breast feeding affect

A

more lactobacillus may reduce candida levels

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9
Q

most common colonisation from mother to child

A

streptococcus saliarius

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10
Q

colonisation sites in the mouth

A
teeth tongue
cheeks, lips, saliva
orthodontic saliva
implants
dentures
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11
Q

what is dental disease caused by

A

mix of species of bacteria working together

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12
Q

formation of plaque biofilm steps

A

host surface adhesion
host surface adhesion (specific)
inter bacterial adhesion
growth and multiplication

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13
Q

describe the first host surface adhesion

A

reversible

physio-chemicla interactions

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14
Q

describe the second host surface adhersion

A

irreversible

specific molecular interctions

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15
Q

interbacterial adhesion

A

co aggregation

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16
Q

how do bacteria adhere to the tooth

A

through the acquirred pellicle

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17
Q

what is the pellicle on the tooth

A

layer of selectively absorbed salivary protiens

forms on the surface enamel

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18
Q

what is found in the pellicle

A

proline rich protien
mucins
statherian

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19
Q

what are the adhesion mechanisms

A

electrostatis
protein carbohydrate/protien-protein
polymer bridging

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20
Q

what are glycoprotiens

A

sugar chains linked by N/O glycosylation to mucins

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21
Q

how can bacteria be attached to glycoprotiens

A

by lectins (lectins target is a sugar on another molecule)

22
Q

what are cryptitiopes

A

hidden receptors for bacteial adhesion

23
Q

how can species of bacteria help each other

A

one uses proteins to remove the sugar exposing the cryptitiope so the other can attach to the exposed region

24
Q

what is it called when bacteria adhere to each other

A

co aggregation

25
Q

what does coaggregation of bacteria involve

A
surface appendages (fimbria or fibrils)
fibrils can grab sugar or protien so the bactiera can stick down
26
Q

how can a microbiome build up

A

bacteria can aggreagate between them
bind to acid of mucins
bind to polysaccharides or surface protiens

27
Q

sources of nutrition for plaque

A

salivery glycoprotiens
salivery protiens
urea

28
Q

how does ph vary in plaque

A

pH lower in lower bifilm

29
Q

how does salivery nutriens vary in biofilm

A

higher closer to top

30
Q

where do aerobic species prefer in the biofilm

A

top

31
Q

what do gradients in the plaque lead to

A

different niches

32
Q

what do autoinducers do

A

signal to increase the density of bacteria where bacteria are most beneficial

33
Q

what can bacteria sense

A

population numbers influencing behavior

34
Q

what happens at high cell density

A

high autoinducer

leads to transcription of target gene

35
Q

what happens at low cell density

A

low autoinducer

no transcription of target gene

36
Q

what is calculus

A

calcified plaque matrix and bacteria

37
Q

how does calculus form

A

increase in pH, Ca and phosphate

mineral growth around bacteria

38
Q

limitations to plaque deveolping

A

salivary flow
gingival crevicular fluid
antibacterial substances

39
Q

antibacterial substances

A

lysosome (breaks down cell walls)
lactoferrin (limits and binds iron)
sialoperoxidase (attack bacteria)
aggreagating factor (stop bacteria attatching to surface)

40
Q

progression of plaque formation steps

A
primary adherer
metabolism
changes environment
bacterial succession
climax community
41
Q

colonisation sites in the mouth

A
teeth
tongue
cheeks/lips/palate
orthdontic appliances
implants
dentures
42
Q

teeth niche

A

calciumhydroxyapitite mineral
stagnation sites have less saliva flow
food source from ofood

43
Q

tongue niche

A

lots of niches due to irregular surface

44
Q

cheeks/lips/saliva niche

A

more saliva present
micro biome more stable, less diverse
dominated by streptococcus

45
Q

denture niche

A

favours fungal growth

46
Q

how to cryotitopes become exposed

A
adsorption to the tooth
enzymic alteration (protease/glucosidase action)
47
Q

building up of a micro biome coloniser

A

actinomyces selectively bind tostatherin and PRPs

strep. sanguimis binds to silica acid of mucisn
strep. mutans binds to PRPs and mucin like glycoprotein

48
Q

inter bacterial substaces

A

salivary material
bacterial exopolysaccharides
bacterial surface structres

49
Q

what can components of interbacteral substances do

A

adsorb to bacterial surfaces
precipitate from solution
chemical change pH

50
Q

what does quorum sensing and autoinducers do

A

allow coordination of behaviour

  • biofilm formation
  • virulence factor producion
  • bioluminesense